Enhanced low-cost lipopeptide biosurfactant production by Bacillus velezensis from residual glycerin.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2024-09-01 Epub Date: 2024-06-25 DOI:10.1007/s00449-024-03051-y

Henrique A Brito, Amanda P Napp, Evandro Pereira, Evelise Bach, João V B Borowski, Luciane M P Passaglia, Vania M M Melo, Raphael Moreira, E Johan Foster, Fernanda C Lopes, Marilene H Vainstein

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Abstract

Biosurfactants (BSFs) are molecules produced by microorganisms from various carbon sources, with applications in bioremediation and petroleum recovery. However, the production cost limits large-scale applications. This study optimized BSFs production by Bacillus velezensis (strain MO13) using residual glycerin as a substrate. The spherical quadratic central composite design (CCD) model was used to standardize carbon source concentration (30 g/L), temperature (34 °C), pH (7.2), stirring (239 rpm), and aeration (0.775 vvm) in a 5-L bioreactor. Maximum BSFs production reached 1527.6 mg/L of surfactins and 176.88 mg/L of iturins, a threefold increase through optimization. Microbial development, substrate consumption, concentration of BSFs, and surface tension were also evaluated on the bioprocess dynamics. Mass spectrometry Q-TOF-MS identified five surfactin and two iturin isoforms produced by B. velezensis MO13. This study demonstrates significant progress on BSF production using industrial waste as a microbial substrate, surpassing reported concentrations in the literature.

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Velezensis 杆菌利用残留甘油生产低成本脂肽生物表面活性剂的能力得到增强。

生物表面活性剂（BSFs）是微生物利用各种碳源产生的分子，可应用于生物修复和石油回收。然而，生产成本限制了其大规模应用。本研究以残余甘油为底物，优化了 Velezensis 杆菌（菌株 MO13）的 BSFs 产量。在 5 升生物反应器中，采用球形二次中央复合设计（CCD）模型对碳源浓度（30 g/L）、温度（34 °C）、pH 值（7.2）、搅拌（239 rpm）和通气（0.775 vvm）进行了标准化。通过优化，BSFs 的最高产量达到了 1527.6 mg/L（表面活性剂）和 176.88 mg/L（伊曲肽），增加了三倍。此外，还评估了微生物发展、底物消耗、BSFs 浓度和表面张力对生物过程动态的影响。质谱 Q-TOF-MS 鉴定出了由 B. velezensis MO13 产生的五种表面活性剂和两种伊图灵同工酶。这项研究表明，利用工业废物作为微生物底物生产 BSF 取得了重大进展，其浓度超过了文献报道的浓度。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.