Glutathione-induced hydrogen sulfide enhances drought tolerance in sweet pepper (Capsicum annuum L.)

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Energy Security Pub Date : 2024-06-14 DOI:10.1002/fes3.559

Cengiz Kaya, Ferhat Uğurlar

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Abstract

Glutathione (GSH) has been studied for its potential to enhance stress tolerance in plant systems, but the role of hydrogen sulfide (H₂S) in GSH-induced water stress tolerance in sweet pepper (Capsicum annuum L.) is still under investigation. This study explores how H₂S and GSH modulate water stress tolerance in pepper plants, addressing a research gap. The joint effect of sodium hydrosulfide (NaHS), a donor of H₂S, and GSH on water stress tolerance was determined through pre-treatment with the H₂S scavenger 0.1 mM hypotaurine (HT). Pepper seedlings were sprayed with 1.0 mM GSH or GSH + 0.2 mM NaHS once a week, with soil moisture content set at 80% and 40% for full irrigation and water stress conditions for a duration of 2 weeks. The results showed that water stress significantly reduced total plant dry weight, chlorophyll a and b content, Fv/Fm, leaf water potential, and relative water content by 50%, 56%, 33%, 27%, 52%, and 34%, while increasing hydrogen peroxide (H₂O₂), proline, and H₂S levels by 152%, 134%, and77%, respectively. Treatment with GSH and NaHS reduced water stress-induced H₂O₂ production and improved plant growth, photosynthetic traits, proline, and the activity of L-cysteine desulfhydrase (L-DES), leading to the generation of H₂S content. GSH reduced NADPH oxidase (NOX), superoxide dismutase (SOD), and H₂O₂ but increased glutathione peroxidase (GPX) activities. The interaction of NaHS and GSH led to further reductions in NOX, SOD, and H₂O₂ values but increased GPX activity. The combined GSH and NaHS treatment increased nitric oxide (NO) production but decreased the activity of S-nitrosoglutathione reductase (GSNOR), potentially accelerating S-nitrosylation. Hypotaurine negated the positive impacts of GSH on water stress tolerance by reducing H₂S concentration in pepper plants, but this was corrected by the concurrent application of NaHS and GSH + HT. Therefore, water stress tolerance requires H₂S.

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谷胱甘肽诱导硫化氢增强甜椒（Capsicum annuum L.）的耐旱性

人们一直在研究谷胱甘肽（GSH）在植物系统中提高胁迫耐受性的潜力，但硫化氢（H2S）在 GSH 诱导的甜椒（Capsicum annuum L.）水胁迫耐受性中的作用仍在研究之中。本研究探讨了硫化氢和 GSH 如何调节辣椒植物的水胁迫耐受性，填补了这一研究空白。通过用 H2S 清除剂 0.1 mM 低牛磺酸（HT）进行预处理，确定了 H2S 供体硫化钠（NaHS）和 GSH 对水分胁迫耐受性的共同影响。辣椒幼苗每周喷洒一次 1.0 mM GSH 或 GSH + 0.2 mM NaHS，土壤含水量设定为 80% 和 40%，分别用于充分灌溉和水胁迫条件，持续 2 周。结果表明，水分胁迫会显著降低植物总干重、叶绿素 a 和 b 含量、Fv/Fm、叶片水势和相对含水量，降幅分别为 50%、56%、33%、27%、52% 和 34%，而过氧化氢（H2O2）、脯氨酸和 H2S 含量则分别增加 152%、134% 和 77%。用 GSH 和 NaHS 处理可减少水胁迫诱导的 H2O2 生成，改善植物生长、光合性状、脯氨酸和 L-半胱氨酸脱硫水解酶（L-DES）的活性，从而产生 H2S 含量。GSH 降低了 NADPH 氧化酶（NOX）、超氧化物歧化酶（SOD）和 H2O2 的活性，但提高了谷胱甘肽过氧化物酶（GPX）的活性。NaHS 和 GSH 的相互作用进一步降低了 NOX、SOD 和 H2O2 的值，但增加了 GPX 的活性。GSH 和 NaHS 的联合处理增加了一氧化氮（NO）的产生，但降低了 S-亚硝基谷胱甘肽还原酶（GSNOR）的活性，从而可能加速 S-亚硝基化。牛磺酸降低了辣椒植株中的 H2S 浓度，从而抵消了 GSH 对水胁迫耐受性的积极影响，但同时施用 NaHS 和 GSH + HT 可以纠正这种情况。因此，水胁迫耐受性需要 H2S。

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来源期刊

Food and Energy Security Energy-Renewable Energy, Sustainability and the Environment

CiteScore

9.30

自引率

4.00%

发文量

审稿时长

19 weeks

期刊介绍： Food and Energy Security seeks to publish high quality and high impact original research on agricultural crop and forest productivity to improve food and energy security. It actively seeks submissions from emerging countries with expanding agricultural research communities. Papers from China, other parts of Asia, India and South America are particularly welcome. The Editorial Board, headed by Editor-in-Chief Professor Martin Parry, is determined to make FES the leading publication in its sector and will be aiming for a top-ranking impact factor. Primary research articles should report hypothesis driven investigations that provide new insights into mechanisms and processes that determine productivity and properties for exploitation. Review articles are welcome but they must be critical in approach and provide particularly novel and far reaching insights. Food and Energy Security offers authors a forum for the discussion of the most important advances in this field and promotes an integrative approach of scientific disciplines. Papers must contribute substantially to the advancement of knowledge. Examples of areas covered in Food and Energy Security include: • Agronomy • Biotechnological Approaches • Breeding & Genetics • Climate Change • Quality and Composition • Food Crops and Bioenergy Feedstocks • Developmental, Physiology and Biochemistry • Functional Genomics • Molecular Biology • Pest and Disease Management • Post Harvest Biology • Soil Science • Systems Biology