Structural basis for odorant recognition of the insect odorant receptor OR-Orco heterocomplex

IF 45.8 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2024-06-13 DOI:10.1126/science.adn6881

Yidong Wang, Liang Qiu, Bing Wang, Zeyuan Guan, Zhi Dong, Jie Zhang, Song Cao, Lulu Yang, Bo Wang, Zhou Gong, Liwei Zhang, Weihua Ma, Zhu Liu, Delin Zhang, Guirong Wang, Ping Yin

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Abstract

Insects detect and discriminate a diverse array of chemicals using odorant receptors (ORs), which are ligand-gated ion channels comprising a divergent odorant-sensing OR and a conserved odorant receptor co-receptor (Orco). In this work, we report structures of the ApOR5-Orco heterocomplex from the pea aphid Acyrthosiphon pisum alone and bound to its known activating ligand, geranyl acetate. In these structures, three ApOrco subunits serve as scaffold components that cannot bind the ligand and remain relatively unchanged. Upon ligand binding, the pore-forming helix S7b of ApOR5 shifts outward from the central pore axis, causing an asymmetrical pore opening for ion influx. Our study provides insights into odorant recognition and channel gating of the OR-Orco heterocomplex and offers structural resources to support development of innovative insecticides and repellents for pest control.

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昆虫气味受体 OR-Orco 异构体识别气味的结构基础

昆虫利用气味受体（ORs）来检测和分辨各种化学物质，气味受体是配体门控离子通道，由不同的气味传感 OR 和保守的气味受体共受体（Orco）组成。在这里，我们报告了豌豆蚜Acyrthosiphon pisum的Ap OR5-Orco异质复合物的单独结构以及与其已知激活配体乙酸香叶酯结合的结构。在这些结构中，三个 Ap Orco 亚基作为支架成分，不能与配体结合，并保持相对不变。配体结合后，Ap OR5 的孔形成螺旋 S7b 从中心孔轴向外移动，导致离子流入的不对称孔开口。我们的研究深入揭示了 OR-Orco 异质复合物的气味识别和通道门控，并为开发创新型杀虫剂和驱虫剂提供了结构资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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