The MATH test. A three-phase assay?

IF 2.2 4区 生物学 Q3 MICROBIOLOGY
Jesús A Salas-Tovar, Sarai Escobedo-García, Guadalupe I Olivas, Carlos H Acosta-Muñiz, Federico Harte, David R Sepulveda
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引用次数: 0

Abstract

This study aimed to investigating the possible interference caused by glass test tubes on the quantification of bacterial adhesion to hydrocarbons by the MATH test. The adhesion of four bacteria to hexadecane and to glass test tubes was evaluated employing different suspending polar phases. The role of the ionic strength of the polar phase regarding adhesion to glassware was investigated. Within the conditions studied, Gram-positive bacteria adhered to both the test tube and the hydrocarbon regardless of the polar phase employed; meanwhile, Escherichia coli ATCC 25922 did not attach to either one. The capacity of the studied microorganisms to adhere to glassware was associated with their electron-donor properties. The ionic strength of the suspending media altered the patterns of adhesion to glass in a strain-specific manner by defining the magnitude of electrostatic repulsion observed between bacteria and the glass surface. This research demonstrated that glass test tubes may interact with suspended bacterial cells during the MATH test under specific conditions, which may lead to overestimating the percentage of adhesion to hydrocarbons and, thus, to erroneous values of cell surface hydrophobicity.

数学测试。三阶段测试?
本研究旨在调查玻璃试管对通过 MATH 试验量化细菌对碳氢化合物的附着力可能造成的干扰。使用不同的悬浮极性相评估了四种细菌对十六烷和玻璃试管的附着力。研究了极性相的离子强度对玻璃器皿附着力的影响。在所研究的条件下,无论采用哪种极性相,革兰氏阳性细菌都能附着在试管和碳氢化合物上;而大肠杆菌 ATCC 25922 则不能附着在任何一种试管和碳氢化合物上。所研究微生物粘附在玻璃器皿上的能力与其电子供体特性有关。悬浮介质的离子强度通过确定细菌与玻璃表面之间的静电斥力大小,以特定的方式改变了菌株对玻璃的粘附模式。这项研究表明,在特定条件下进行 MATH 试验时,玻璃试管可能会与悬浮的细菌细胞相互作用,这可能会导致高估碳氢化合物的粘附百分比,从而导致细胞表面疏水性的错误值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Fems Microbiology Letters
Fems Microbiology Letters 生物-微生物学
CiteScore
4.30
自引率
0.00%
发文量
112
审稿时长
1.9 months
期刊介绍: FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.
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