Effect of loading rate and pH on glycerol fermentation and microbial population in an upflow anaerobic filter reactor.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2024-07-01 Epub Date: 2024-06-01 DOI:10.1007/s00449-024-03003-6

Cândida N Cordeiro, Patricia Rojas, Shyrlane T S Veras, Mario T Kato, Lourdinha Florencio, José Luis Sanz

{"title":"Effect of loading rate and pH on glycerol fermentation and microbial population in an upflow anaerobic filter reactor.","authors":"Cândida N Cordeiro, Patricia Rojas, Shyrlane T S Veras, Mario T Kato, Lourdinha Florencio, José Luis Sanz","doi":"10.1007/s00449-024-03003-6","DOIUrl":null,"url":null,"abstract":"A reactor with silicone tubes as support medium was used for glycerol fermentation. The experimental set-up consisted of three phases. In P1, the applied glycerol loading rate (gly-LR) was in the range of 6-10 g.L-1.d-1 at an influent pH of 7.9 ± 0.4. In P2, gly-LR was kept constant (18.0 ± 1.8 g.L-1.d-1) with different doses of NaHCO3. Finally in P3, two different gly-LR (9 and 18 g.L-1.d-1) were evaluated, dosing 1 g-NaHCO3 per g-COD of glycerol. Glycerol consumption was close 90%. The main end-product was 1,3-propanediol (1,3-PDO) (0.40 mol.mol-gly-1), but ethanol was also generated, particularly at pH above 8 and low gly-LR (0.20 mol.mol-gly-1). After 1-year operation with glycerol as the only carbon source, a drastic shift in the bacterial community was observed. The 1,3-PDO producers Lacrimispora and Clostridium became dominant, although non-glycerol-degrading fermentative genera, e.g., Actinomyces and Eubacterium, thrived at the expense of cellular breakdown products.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"991-1002"},"PeriodicalIF":3.5000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11213801/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprocess and Biosystems Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s00449-024-03003-6","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/6/1 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

A reactor with silicone tubes as support medium was used for glycerol fermentation. The experimental set-up consisted of three phases. In P1, the applied glycerol loading rate (gly-LR) was in the range of 6-10 g.L^-1.d^-1 at an influent pH of 7.9 ± 0.4. In P2, gly-LR was kept constant (18.0 ± 1.8 g.L^-1.d^-1) with different doses of NaHCO₃. Finally in P3, two different gly-LR (9 and 18 g.L^-1.d^-1) were evaluated, dosing 1 g-NaHCO₃ per g-COD of glycerol. Glycerol consumption was close 90%. The main end-product was 1,3-propanediol (1,3-PDO) (0.40 mol.mol-gly^-1), but ethanol was also generated, particularly at pH above 8 and low gly-LR (0.20 mol.mol-gly^-1). After 1-year operation with glycerol as the only carbon source, a drastic shift in the bacterial community was observed. The 1,3-PDO producers Lacrimispora and Clostridium became dominant, although non-glycerol-degrading fermentative genera, e.g., Actinomyces and Eubacterium, thrived at the expense of cellular breakdown products.

Abstract Image

查看原文本刊更多论文

负载率和 pH 值对上流式厌氧过滤反应器中甘油发酵和微生物数量的影响。

甘油发酵采用硅胶管作为支撑介质的反应器。实验装置包括三个阶段。在 P1 阶段，进水 pH 值为 7.9 ± 0.4，甘油负载率（gly-LR）为 6-10 g.L-1.d-1。在 P2 中，不同剂量的 NaHCO3 使 gly-LR 保持不变（18.0 ± 1.8 g.L-1.d-1）。最后，在 P3 中，评估了两种不同的 gly-LR（9 和 18 g.L-1.d-1），每 gCOD甘油添加 1 g-NaHCO3。甘油消耗量接近 90%。主要的最终产品是 1,3-丙二醇（1,3-PDO）（0.40 mol.mol-gly-1），但也会产生乙醇，特别是在 pH 值高于 8 和甘油-LR 较低时（0.20 mol.mol-gly-1）。在以甘油为唯一碳源的条件下运行 1 年后，观察到细菌群落发生了急剧变化。1,3-PDO 生产者 Lacrimispora 和 Clostridium 成为主导，而非甘油降解发酵菌属，如放线菌和放线杆菌，则以牺牲细胞分解产物为代价而茁壮成长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.