Untargeted metabolomics, optimization of microwave-assisted extraction using Box-Behnken design and evaluation of antioxidant, and antidiabetic activities of sugarcane bagasse.

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Phytochemical Analysis Pub Date : 2024-10-01 Epub Date: 2024-05-30 DOI:10.1002/pca.3395
Chitchamai Ovatlarnporn, Abdul Basit, Himanshu Paliwal, Sirinporn Nalinbenjapun, Sasikarn Sripetthong, Acharee Suksuwan, Nureesun Mahamud, Opeyemi Joshua Olatunji
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引用次数: 0

Abstract

Introduction: The fruit wastes, in particular agricultural wastes, are considered potential and inexpensive sources of bioactive compounds.

Objective: The current study was aimed at the preparation of an optimized extract of sugarcane bagasse using microwave-assisted extraction (MAE) technology and comparative evaluation of chemical composition, antioxidant, and antidiabetic activities with extract prepared through maceration technique.

Methodology: Box-Behnken Design (BDD) with response surface methodology was applied to observe interactions of three independent variables (ethanol concentrations [%], microwave power [W], and extraction time [min]) on the dependent variables (total phenolic content [TPC] and antioxidant status via 2,2-diphenyl-1-picrylhydrazyl [DPPH] to establish optimal extraction conditions. The ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) analysis was applied for untargeted metabolite profiling, and in vitro assays were used for evaluation of the antidiabetic and antioxidant potential of the extract. Moreover, an in silico study was used to predict the interaction of five dominant compounds from the UHPLC-Q-TOF-MS profile against the dipeptidyl peptidase-IV (DPP-IV) enzyme.

Results: The optimal conditions for the extraction were established at 60% (v/v) ethanol, 500 W microwave power, and 5 min time with TPC 12.83 ± 0.66 mg GAE/g d.w. and DPPH 45.09 ± 0.07%. The UHPLC-Q-TOF-MS analysis revealed the presence of a total of 106 compounds in the extract. Moreover, the extract prepared through MAE technology presented higher TPC and DPPH findings than the extract prepared through maceration. Similarly, the extract was also found with good antidiabetic activity by inhibiting the DPP-IV enzyme which was also rectified theoretically by a molecular docking study.

Conclusion: The current study presents a sustainable and an optimized approach for the preparation of sugarcane bagasse extract with functional phytoconstituents and higher antidiabetic and antioxidant activities.

非靶向代谢组学、使用 Box-Behnken 设计优化微波辅助萃取以及甘蔗渣抗氧化和抗糖尿病活性评估。
简介:水果废弃物,特别是农业废弃物,被认为是潜在的生物活性化合物的廉价来源:水果废料,尤其是农业废料,被认为是生物活性化合物的潜在廉价来源:本研究旨在利用微波辅助萃取(MAE)技术制备甘蔗渣的优化提取物,并对其化学成分、抗氧化性和抗糖尿病活性与浸渍技术制备的提取物进行比较评估:采用箱-贝肯设计(BDD)和响应面方法观察三个自变量(乙醇浓度[%]、微波功率[瓦]和萃取时间[分])对因变量(总酚含量[TPC]和通过2,2-二苯基-1-苦基肼[DPPH]检测的抗氧化状态)的交互作用,以确定最佳萃取条件。超高效液相色谱-四极杆飞行时间质谱(UHPLC-Q-TOF-MS)分析用于非目标代谢物分析,体外检测用于评估提取物的抗糖尿病和抗氧化潜力。此外,还采用了一项硅学研究来预测 UHPLC-Q-TOF-MS 图谱中五种主要化合物与二肽基肽酶-IV(DPP-IV)酶的相互作用:最佳萃取条件为 60% (v/v) 乙醇、500 W 微波功率和 5 分钟时间,TPC 为 12.83 ± 0.66 mg GAE/g d.w.,DPPH 为 45.09 ± 0.07%。UHPLC-Q-TOF-MS 分析显示,提取物中共含有 106 种化合物。此外,通过 MAE 技术制备的提取物的 TPC 和 DPPH 结果均高于浸渍法制备的提取物。同样,还发现该提取物通过抑制 DPP-IV 酶具有良好的抗糖尿病活性,分子对接研究也从理论上纠正了这一点:目前的研究提出了一种可持续的优化方法,用于制备具有功能性植物成分和较高抗糖尿病和抗氧化活性的甘蔗渣提取物。
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来源期刊
Phytochemical Analysis
Phytochemical Analysis 生物-分析化学
CiteScore
6.00
自引率
6.10%
发文量
88
审稿时长
1.7 months
期刊介绍: Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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