Multifaceted Chemico-Pharmacological Insights into Cynometra ramiflora L.: Unveiling its GC-MS, Cytotoxic, Thrombolytic, Anti-Inflammatory, Antioxidant, Anti-Diarrheal, Hypoglycemic, and Analgesic Potentials

IF 1.5 4区医学 Q4 CHEMISTRY, MEDICINAL

Natural Product Communications Pub Date : 2024-05-27 DOI:10.1177/1934578x241257377

Md. Abdus Samadd, Md. Rifaet Hossain, Mohammad Abdullah Taher, Mohammad Mahmudul Hasan, Shariful Haque, Mashruf Islam Rafid, Md. Solaiman Hossain, Md. Ashraful Islam, Mala Khan

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引用次数: 0

Abstract

Objective: This study aimed to comprehensively evaluate the chemico-pharmacological properties of methanolic extractives derived from Cyanometra ramiflora leaves, encompassing a spectrum of activities including cytotoxic, thrombolytic, membrane-stabilizing, antioxidant, antidiarrheal, hypoglycemic, and analgesic effects. Methods: Phytomolecules were identified using Gas Chromatography-Mass Spectrometry (GC-MS), while antioxidant activity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and total phenolic content tests. Cytotoxicity was determined through the brine shrimp bioassay, while thrombolytic activity was evaluated via clot lysis assays, and membrane-stabilizing effects were investigated for potential anti-inflammatory properties. In-vivo experiments using mice were conducted solely with the methanolic extract to assess central and peripheral analgesic, hypoglycemic, and antidiarrheal properties. Results: The presence of 47 compounds in the methanolic extract, with tetratriacontyl pentafluoropropionate (9.25%), pentatriacontane (8.75%), and 2-methylhexacosane (5.9%) being predominant. The dichloromethane-soluble fraction exhibited the highest cytotoxicity, with a 50% lethal concentration (LC50) of 11.05 µg/mL. Both petroleum-ether and methanol-soluble fractions demonstrated more than 50% lysis in thrombolytic activity assays. Under hypotonic and heat-induced conditions, the ethyl acetate and dichloromethane-soluble fractions of C. ramiflora significantly reduced erythrocyte hemolysis. Notably, the methanol-soluble fraction exhibited significant antioxidant potential with 50% inhibitory concentration (IC50) of 17.71 μg/mL compared to the standard EDTA (IC50 = 75.94 μg/mL). The hypoglycemic investigation revealed a 63.73% reduction at 90 min with a 600 mg/kg dose, while mild antidiarrheal activity was observed with a 37.5% reduction in diarrhea at 4 h intervals. Significant analgesic effects were demonstrated in both central and peripheral tests, with a 68.53% reduction in writhing during the peripheral test and an average tail immersion time of 5.38 s at 90 min. Conclusion: C. ramiflora leaves show promise as natural sources for various pharmacological activities, including cytotoxicity, thrombosis, inflammation, antioxidant, hypoglycemic, and analgesic effects. However, further comprehensive research is recommended to elucidate the precise mechanisms of action and analyze the phytochemical composition of C. ramiflora plants.

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Cynometra ramiflora L.的多方面化学药理学见解：揭示其 GC-MS、细胞毒性、溶栓、抗炎、抗氧化、止泻、降糖和镇痛潜力

研究目的本研究旨在全面评估从蓝花楹叶中提取的甲醇萃取物的化学药理特性，包括细胞毒性、血栓溶解、膜稳定、抗氧化、止泻、降血糖和镇痛作用。方法：使用气相色谱-质谱法（GC-MS）鉴定植物大分子，使用 2,2-二苯基-1-苦基肼（DPPH）和总酚含量测试评估抗氧化活性。通过盐水虾生物测定法测定了细胞毒性，通过血凝块溶解试验评估了血栓溶解活性，并研究了潜在的抗炎特性的膜稳定作用。仅使用甲醇提取物对小鼠进行了体内实验，以评估中枢和外周镇痛、降血糖和止泻特性。实验结果甲醇提取物中含有 47 种化合物，其中主要是五氟丙酸四叔聪酯（9.25%）、五三聪烷（8.75%）和 2-甲基六六六烷（5.9%）。二氯甲烷可溶部分的细胞毒性最高，50%致死浓度（LC50）为 11.05 微克/毫升。在溶栓活性试验中，石油醚和甲醇溶性馏分的溶解度均超过 50%。在低渗和热诱导条件下，苧麻的乙酸乙酯和二氯甲烷可溶性馏分可显著减少红细胞溶血。值得注意的是，与标准 EDTA（IC50 = 75.94 μg/mL）相比，甲醇可溶性馏分具有明显的抗氧化潜力，其 50％抑制浓度（IC50）为 17.71 μg/mL。降血糖研究显示，600 毫克/千克的剂量可在 90 分钟内降低 63.73%的血糖，而轻微的止泻活性可在 4 小时内减少 37.5%的腹泻。在中枢和外周测试中均显示出显著的镇痛效果，外周测试中蠕动减少了 68.53%，90 分钟时的平均尾部浸泡时间为 5.38 秒。结论苧麻叶片有望成为多种药理活性的天然来源，包括细胞毒性、血栓形成、炎症、抗氧化、降血糖和镇痛作用。不过，建议进一步开展综合研究，以阐明其确切的作用机制，并分析苧麻植物的植物化学成分。

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来源期刊

Natural Product Communications 工程技术-食品科技

CiteScore

3.10

自引率

11.10%

发文量

254

审稿时长

2.7 months

期刊介绍： Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products. Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products. Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products.