{"title":"Exogenous dopamine mitigates the effects of salinity stress in tomato seedlings by alleviating the oxidative stress and regulating phytohormones","authors":"Ertan Yildirim, Melek Ekinci, Metin Turan, Merve Yuce, Selda Ors, Oğuzhan Araz, Umit Torun, Sanem Argin","doi":"10.1007/s11738-024-03656-6","DOIUrl":null,"url":null,"abstract":"<div><p>Salt stress is a worldwide major threat to agricultural production. The aim was to investigate the effects of exogenous dopamine (DA) treatments on physiological, morphological and biochemical characteristics of tomato seedlings under salinity stress. Salt stress was created using a 100 mM NaCl solution. Dopamine solutions (0, 50, 100 and 200 µM) were applied with 7-day intervals. Salt stress significantly suppressed plant growth and DA treatments alleviated the negative effects of salt stress on the growth of tomato seedlings. 100 µM DA treatment increased plant and root dry weights, plant stem diameter, plant height and, leaf area by 286.84%, 150.00%, 108.37%, 160.89%, and 158.28%, respectively, compared to the control. Under salinity LRWC, SPAD, chl-a, chl-b, and total chlorophyll contents decreased; membrane permeability (MP), H<sub>2</sub>O<sub>2</sub>, MDA, proline and sucrose contents, CAT, POD and SOD activities increased. Under salt stress, when 100 µM DA was applied, LRWC, SPAD, chl-a, chl-b, and total chlorophyll contents of plants increased by 13.64%, 18.62%, 43.08%, 64.90%, and 50.00%, while MP reduced by 21.08% compared to the control. When 200 µM DA was applied under salt stress, H<sub>2</sub>O<sub>2</sub>, MDA, proline and sucrose contents, and CAT, POD and SOD activities were reduced by 31.86%, 18.66%, 56.00%, 38.24%, 11.16%, 17.81% and 10.80%, respectively, compared to non-DA-treated plants. Exogenous application of DA increased IAA content, decreased ABA content and increased ratio of K<sup>+</sup>/Na<sup>+</sup> and Ca<sup>2+</sup>/Na<sup>+</sup> under salt stress as well. In conclusion, exogenous dopamine treatments effectively prevent cellular damage in tomato seedlings and improve plant tolerance to salt stress.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"46 5","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11738-024-03656-6.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Physiologiae Plantarum","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s11738-024-03656-6","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Salt stress is a worldwide major threat to agricultural production. The aim was to investigate the effects of exogenous dopamine (DA) treatments on physiological, morphological and biochemical characteristics of tomato seedlings under salinity stress. Salt stress was created using a 100 mM NaCl solution. Dopamine solutions (0, 50, 100 and 200 µM) were applied with 7-day intervals. Salt stress significantly suppressed plant growth and DA treatments alleviated the negative effects of salt stress on the growth of tomato seedlings. 100 µM DA treatment increased plant and root dry weights, plant stem diameter, plant height and, leaf area by 286.84%, 150.00%, 108.37%, 160.89%, and 158.28%, respectively, compared to the control. Under salinity LRWC, SPAD, chl-a, chl-b, and total chlorophyll contents decreased; membrane permeability (MP), H2O2, MDA, proline and sucrose contents, CAT, POD and SOD activities increased. Under salt stress, when 100 µM DA was applied, LRWC, SPAD, chl-a, chl-b, and total chlorophyll contents of plants increased by 13.64%, 18.62%, 43.08%, 64.90%, and 50.00%, while MP reduced by 21.08% compared to the control. When 200 µM DA was applied under salt stress, H2O2, MDA, proline and sucrose contents, and CAT, POD and SOD activities were reduced by 31.86%, 18.66%, 56.00%, 38.24%, 11.16%, 17.81% and 10.80%, respectively, compared to non-DA-treated plants. Exogenous application of DA increased IAA content, decreased ABA content and increased ratio of K+/Na+ and Ca2+/Na+ under salt stress as well. In conclusion, exogenous dopamine treatments effectively prevent cellular damage in tomato seedlings and improve plant tolerance to salt stress.
期刊介绍:
Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry.
The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.