Zebrafish as a model for the study of wound healing in hyperglycemia

IF 0.5 Q4 EDUCATION, SCIENTIFIC DISCIPLINES
Lia Nurkhasanah, Farida Hayati, R. Istikharah
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引用次数: 0

Abstract

Background: For preclinical studies of diabetic wound therapy, zebrafish have many orthologous signalling pathways that play essential roles in wound healing and regeneration. Objectives: This study compares the expression of four essential wound-healing genes and caudal fin regeneration in hyperglycemic zebrafish (Danio rerio) to normal zebrafish. Methods: Hyperglycemia was induced by using the intraperitoneal injection method with 350 mg/BW streptozotocin on days one, three, and five. The regeneration of the zebrafish's caudal fin was observed on day 5 after amputation using a stereomicroscope, followed by sampling of the blastema to analyse gene expression. Caudal fin regeneration was analysed using Zeiss Zen 3.3 blue documentation; blood glucose levels were measured using a glucometer; and relative gene expression analysis of sonic hedgehog (shh), insulin-like growth factor 2a (igf2a), bone morphogenetic protein 2b (bmp2b), collagen 1a2 (col1a2) was performed by qRT-PCR method. Results: The glucose level in the hyperglycemia group was 203.2 mg/dL, and that in the normal group was 59.5 mg/dL. The authors found that igf2a, shh, bmp2b, and col1a2 expression were all downregulated in the hyperglycemia group and decreased in caudal fin regeneration. Conclusion: This novel adult zebrafish model of hyperglycemia significantly impairs gene expression and caudal fin regeneration.
将斑马鱼作为研究高血糖状态下伤口愈合的模型
背景:对于糖尿病伤口治疗的临床前研究,斑马鱼有许多在伤口愈合和再生中发挥重要作用的同源信号通路:本研究比较了高血糖斑马鱼(Danio rerio)和正常斑马鱼的四个重要伤口愈合基因的表达和尾鳍再生。在斑马鱼尾鳍截肢后的第 5 天用体视显微镜观察其再生情况,然后取样分析基因表达。使用 Zeiss Zen 3.3 蓝色记录仪分析尾鳍再生情况;使用血糖仪测量血糖水平;使用 qRT-PCR 方法分析声刺猬(shh)、胰岛素样生长因子 2a(igf2a)、骨形态发生蛋白 2b(bmp2b)、胶原 1a2(col1a2)的相对基因表达情况:结果:高血糖组的血糖水平为 203.2 mg/dL,正常组为 59.5 mg/dL。作者发现,在高血糖组中,igf2a、shh、bmp2b和col1a2的表达均下调,尾鳍再生能力下降:结论:这种新型的成年斑马鱼高血糖模型会严重影响基因表达和尾鳍再生。
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来源期刊
Pharmacy Education
Pharmacy Education EDUCATION, SCIENTIFIC DISCIPLINES-
CiteScore
0.80
自引率
20.00%
发文量
174
期刊介绍: Pharmacy Education journal provides a research, development and evaluation forum for communication between academic teachers, researchers and practitioners in professional and pharmacy education, with an emphasis on new and established teaching and learning methods, new curriculum and syllabus directions, educational outcomes, guidance on structuring courses and assessing achievement, and workforce development. It is a peer-reviewed online open access platform for the dissemination of new ideas in professional pharmacy education and workforce development. Pharmacy Education supports Open Access (OA): free, unrestricted online access to research outputs. Readers are able to access the Journal and individual published articles for free - there are no subscription fees or ''pay per view'' charges. Authors wishing to publish their work in Pharmacy Education do so without incurring any financial costs.
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