Argyreia nervosa-driven biosynthesis of Cu-Ag bimetallic nanoparticles from plant leaves extract unveils enhanced antibacterial properties.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2024-08-01 Epub Date: 2024-05-03 DOI:10.1007/s00449-024-03020-5

Parvathalu Kalakonda, Rajitha Kathi, Merlinsheeba Gali Ligory, Naveenkumar Dabbeta, Naveenkumar Madipoju, Soujanyalakshmi Mynepally, Vijay Morampudi, Sreenivas Banne, Pritam Mandal, Ramu Naidu Savu, Sarvani Jowhar Khanam, Murali Banavoth, Naina Vinodini Sudarsanam Eve, Bala Bhaskar Podila

{"title":"Argyreia nervosa-driven biosynthesis of Cu-Ag bimetallic nanoparticles from plant leaves extract unveils enhanced antibacterial properties.","authors":"Parvathalu Kalakonda, Rajitha Kathi, Merlinsheeba Gali Ligory, Naveenkumar Dabbeta, Naveenkumar Madipoju, Soujanyalakshmi Mynepally, Vijay Morampudi, Sreenivas Banne, Pritam Mandal, Ramu Naidu Savu, Sarvani Jowhar Khanam, Murali Banavoth, Naina Vinodini Sudarsanam Eve, Bala Bhaskar Podila","doi":"10.1007/s00449-024-03020-5","DOIUrl":null,"url":null,"abstract":"Our study specifically explores the biosynthesis of copper-silver bimetallic nanoparticles (Cu-Ag BMNPs) using Argyreia nervosa (AN) plant leaf green extract as a versatile agent for capping, reducing, and stabilizing. This biosynthesis method is characterized by its simplicity and cost-effectiveness, utilizing silver nitrate (AgNO3) and cupric oxide (CuO) as precursor materials. Our comprehensive characterization of the Cu-Ag BMNPs, employing techniques such as X-ray diffraction (XRD), UV-Vis spectrometry, scanning electron microscopy (SEM), Zetasizer, and Fourier transformed infrared spectrometry (FTIR). FTIR analysis reveals biofunctional groups and chemical bands, while SEM and XRD analyses provide morphological and structural details. To evaluate the antimicrobial properties of the Cu-Ag BMNPs, we conducted disc diffusion and minimum inhibitory concentration (MIC) assays against Escherichia coli (E. coli), with results compared to the standard gentamicin antibiotic. It is observed that the 2% and 5% CuO concentrations of AN Cu-Ag BMNPs exhibit substantial antibacterial activity in comparison to AN extract when tested on EPEC. Among these, the Cu-Ag BMNPs at a 2% concentration demonstrate higher antibacterial activity, potentially attributed to the enhanced dispersion of BMNPs facilitated by the lower CuO doping concentration. These two assays showcased the improved antimicrobial activity of Cu-Ag BMNPs, highlighting their synergistic effect, characterized by high MIC values and a broad zone of inhibition in the disc diffusion tests against E. coli. These results emphasize the significant antibacterial potential of the synthesized BMNPs, with a medicinal plant AN leaf extract playing a pivotal role in enhancing antibacterial activity.","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprocess and Biosystems Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s00449-024-03020-5","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/3 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Our study specifically explores the biosynthesis of copper-silver bimetallic nanoparticles (Cu-Ag BMNPs) using Argyreia nervosa (AN) plant leaf green extract as a versatile agent for capping, reducing, and stabilizing. This biosynthesis method is characterized by its simplicity and cost-effectiveness, utilizing silver nitrate (AgNO₃) and cupric oxide (CuO) as precursor materials. Our comprehensive characterization of the Cu-Ag BMNPs, employing techniques such as X-ray diffraction (XRD), UV-Vis spectrometry, scanning electron microscopy (SEM), Zetasizer, and Fourier transformed infrared spectrometry (FTIR). FTIR analysis reveals biofunctional groups and chemical bands, while SEM and XRD analyses provide morphological and structural details. To evaluate the antimicrobial properties of the Cu-Ag BMNPs, we conducted disc diffusion and minimum inhibitory concentration (MIC) assays against Escherichia coli (E. coli), with results compared to the standard gentamicin antibiotic. It is observed that the 2% and 5% CuO concentrations of AN Cu-Ag BMNPs exhibit substantial antibacterial activity in comparison to AN extract when tested on EPEC. Among these, the Cu-Ag BMNPs at a 2% concentration demonstrate higher antibacterial activity, potentially attributed to the enhanced dispersion of BMNPs facilitated by the lower CuO doping concentration. These two assays showcased the improved antimicrobial activity of Cu-Ag BMNPs, highlighting their synergistic effect, characterized by high MIC values and a broad zone of inhibition in the disc diffusion tests against E. coli. These results emphasize the significant antibacterial potential of the synthesized BMNPs, with a medicinal plant AN leaf extract playing a pivotal role in enhancing antibacterial activity.

查看原文本刊更多论文

从植物叶提取物中提取 Argyreia nervosa 驱动的铜银双金属纳米粒子的生物合成揭示了增强的抗菌特性。

我们的研究特别探讨了以神经箭毒（AN）植物叶绿萃取物作为封端、还原和稳定的多功能制剂，生物合成铜银双金属纳米粒子（Cu-Ag BMNPs）的方法。利用硝酸银（AgNO3）和氧化铜（CuO）作为前驱体材料，这种生物合成方法的特点是简单、成本效益高。我们采用 X 射线衍射 (XRD)、紫外可见光谱、扫描电子显微镜 (SEM)、Zetasizer 和傅立叶变换红外光谱 (FTIR) 等技术对铜银 BMNPs 进行了全面表征。傅立叶变换红外光谱分析揭示了生物功能基团和化学带，而扫描电子显微镜和 X 射线衍射分析则提供了形态和结构细节。为了评估铜银 BMNPs 的抗菌特性，我们对大肠杆菌（E. coli）进行了盘扩散和最小抑菌浓度（MIC）检测，并将结果与标准庆大霉素抗生素进行了比较。结果表明，在对 EPEC 进行测试时，与 AN 提取物相比，2% 和 5% CuO 浓度的 AN Cu-Ag BMNPs 具有很强的抗菌活性。其中，浓度为 2% 的铜银 BMNPs 表现出更高的抗菌活性，这可能是由于较低的氧化铜掺杂浓度促进了 BMNPs 的分散。这两项检测结果表明，铜银 BMNPs 的抗菌活性得到了提高，突出了它们的协同效应，其特点是对大肠杆菌的盘扩散试验中具有较高的 MIC 值和较宽的抑制区。这些结果表明，合成的 BMNPs 具有显著的抗菌潜力，而药用植物 AN 叶提取物在增强抗菌活性方面发挥了关键作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.