Acute toxicity test of tablets containing α-mangosteen, piperine, curcumin, methyl cinnamate and vitamin C in female wistar rats

Abstract

Background: Traditional medicine has long been used and contributed to health services. Supplement tablets containing isolated -mangosteen, piperine, curcumin, methyl cinnamate, and vitamin C are potential antivirals. Objective: This study determined the LD50 of these supplement tablets as well as their influence on the liver, stomach, kidneys, spleen, and lungs after the acute oral administration of 5000 mg/kg to female Wistar rats, according to the OECD 425 Up and Down Procedure. Method: The test animals were observed for signs of toxicity for 14 days, the first 48 hours, with observation especially in the first 4 hours every 30 minutes. Results: They showed no signs of toxicity and non-irritating death and had an LD50 toxicity value >5000 mg/kg body weight. Therefore, the tablets were deemed practically non-toxic. Histopathology revealed no changes in the liver, spleen, and lungs, however desquamation and ulceration of the stomach and glomerular degeneration and atrophy in the kidney were light and reversible. Conclusion: The tablets containing isolated α-mangosteen, piperine, curcumin, methyl cinnamate and vitamin C were practically non-toxic.