Elimination of zero-repeat subunit in allergenic seed protein 13S globulin using the novel allele GlbNB2 in common buckwheat (Fagopyrum esculentum Moench)

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY
Takeyuki Okada, Kohtaro Kimura, Naruha Goto, Tomoyuki Katsube-Tanaka
{"title":"Elimination of zero-repeat subunit in allergenic seed protein 13S globulin using the novel allele GlbNB2 in common buckwheat (Fagopyrum esculentum Moench)","authors":"Takeyuki Okada,&nbsp;Kohtaro Kimura,&nbsp;Naruha Goto,&nbsp;Tomoyuki Katsube-Tanaka","doi":"10.1016/j.fochms.2024.100205","DOIUrl":null,"url":null,"abstract":"<div><p>Common buckwheat (<em>Fagopyrum esculentum</em> Moench) seeds contain 13S globulin, the zero-repeat subunit of which is trypsin-resistant and allergenic. Here, its two novel alleles were analyzed for development of hypoallergenic plants. The <em>GlbNC</em> allele has a Miniature Inverted-repeat Transposable Element (MITE)-like insertion in the 4th exon. However, most of the insertion was spliced-out, resulting in accumulation of zero-repeat subunit in <em>GlbNC</em> homozygotes. Meanwhile, the <em>GlbNB2</em> has a 164-bp insertion in the 3rd exon, resulting in no accumulation of zero-repeat subunit in <em>GlbNB2</em> homozygotes (NB2_homo). Both the insertion sequences were predicted to form a hairpin-like structure, and that of <em>GlbNB2</em> was more rigid than that of <em>GlbNC</em>. Trypsin digestion in NB2_homo showed that the α polypeptide of Met-rich subunit is also hard to digest, that is a next target to eliminate for hypoallergenic buckwheat development.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":null,"pages":null},"PeriodicalIF":4.1000,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666566224000121/pdfft?md5=1ec92eae80cad5ebbf8879b82264d3a0&pid=1-s2.0-S2666566224000121-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry Molecular Sciences","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666566224000121","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Common buckwheat (Fagopyrum esculentum Moench) seeds contain 13S globulin, the zero-repeat subunit of which is trypsin-resistant and allergenic. Here, its two novel alleles were analyzed for development of hypoallergenic plants. The GlbNC allele has a Miniature Inverted-repeat Transposable Element (MITE)-like insertion in the 4th exon. However, most of the insertion was spliced-out, resulting in accumulation of zero-repeat subunit in GlbNC homozygotes. Meanwhile, the GlbNB2 has a 164-bp insertion in the 3rd exon, resulting in no accumulation of zero-repeat subunit in GlbNB2 homozygotes (NB2_homo). Both the insertion sequences were predicted to form a hairpin-like structure, and that of GlbNB2 was more rigid than that of GlbNC. Trypsin digestion in NB2_homo showed that the α polypeptide of Met-rich subunit is also hard to digest, that is a next target to eliminate for hypoallergenic buckwheat development.

Abstract Image

利用普通荞麦(Fagopyrum esculentum Moench)中的新型等位基因 GlbNB2 消除致敏种子蛋白 13S 球蛋白中的零重复亚基
普通荞麦(Fagopyrum esculentum Moench)种子含有 13S 球蛋白,其零重复亚基具有抗胰蛋白酶和致敏性。在此,对其两个新型等位基因进行了分析,以开发低过敏性植物。GlbNC 等位基因的第 4 个外显子上有一个类似于微型反向重复可转座元件(MITE)的插入。然而,大部分插入片段被剪接掉了,导致零重复亚基在 GlbNC 等位基因中积累。与此同时,GlbNB2 的第 3 个外显子上有一个 164 bp 的插入片段,导致在 GlbNB2 同源型基因(NB2_homo)中没有零重复亚基的积累。据预测,这两个插入序列都会形成发夹状结构,而 GlbNB2 的结构比 GlbNC 的结构更为坚硬。胰蛋白酶消化 NB2_homo 的结果表明,富含 Met 的亚基的 α 多肽也很难被消化,这是开发低过敏性荞麦的下一个目标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Food Chemistry Molecular Sciences
Food Chemistry Molecular Sciences Agricultural and Biological Sciences-Food Science
CiteScore
6.00
自引率
0.00%
发文量
83
审稿时长
82 days
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信