Preparation and evaluation of sunflower oil by enzymatic aqueous extraction

IF 1.9 4区 农林科学 Q3 CHEMISTRY, APPLIED
He Huang, Baohui Pan, Xiaoming Jiang, Shu Wang, Jingting Guo, Pan Gao, Wu Zhong, Chuanrong Hu, Dongping He, Jiaojiao Yin
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Abstract

The objective of this study was to produce sunflower oil using pectinase, flavor protease, and alkaline protease. The optimal parameters for the enzymatic hydrolysis process were determined through sensory evaluation, resulting in a temperature of 120°C, a duration of 30 minutes, a polysaccharide to protein ratio of 2:1 for the enzymatic hydrolysate, and an enzymatic hydrolysate to oil ratio of 1:3. Under these specific conditions, the sunflower oil (K4) achieved the highest sensory score of 13.9, exhibiting a pronounced oily flavor alongside moderate baked and nutty flavors. Compared to the controls of first-grade sunflower oil (K1), sunflower kernel oil (K2) and sunflower oil prepared by roasting (K3), the acid value (AV) in K4 exhibited a statistically significant decrease compared to K2 and K3, conversely, the peroxide value (PV) and BaP content in K4 demonstrated a statistically significant increase compared to K1 and K2. Additionally, the tocopherols in K4 exhibited a lower concentration compared to K1, while the sterols were effectively retained and showed no significant difference to these control groups. The fatty acid composition of the four different sunflower oil samples were analyzed, revealing that the contents of oleic acid (C18:1) and monounsaturated fatty acid (MUFA) in K4 were significantly higher than K2, and there was no significant difference compared to K1. This investigation of the present study could provide a certain theoretical basis for the production of sunflower oil by enzymes.

Abstract Image

Abstract Image

通过酶法水提取制备和评估葵花籽油
本研究的目的是利用果胶酶、风味蛋白酶和碱性蛋白酶生产葵花籽油。通过感官评估确定了酶水解过程的最佳参数,温度为 120°C,持续时间为 30 分钟,酶水解物的多糖与蛋白质比例为 2:1,酶水解物与油的比例为 1:3。在这些特定条件下,葵花籽油(K4)的感官评分最高,达到 13.9 分,表现出明显的油脂味以及适度的烘焙味和坚果味。与对照组一级葵花籽油(K1)、葵花籽仁油(K2)和通过烘烤制备的葵花籽油(K3)相比,K4 的酸值(AV)在统计学上显著低于 K2 和 K3,相反,K4 的过氧化值(PV)和 BaP 含量在统计学上显著高于 K1 和 K2。此外,与 K1 相比,K4 中生育酚的浓度较低,而固醇则得到了有效保留,与这些对照组相比没有明显差异。对四种不同葵花籽油样品的脂肪酸组成进行分析后发现,K4 中油酸(C18:1)和单不饱和脂肪酸(MUFA)的含量明显高于 K2,与 K1 相比没有明显差异。本研究的这一调查可为酶法生产葵花籽油提供一定的理论依据。
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来源期刊
CiteScore
4.10
自引率
5.00%
发文量
95
审稿时长
2.4 months
期刊介绍: The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate. JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of year­to­year, environmental, and/ or cultivar variations through use of appropriate statistical analyses.
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