Metal-chelating antioxidant peptides: Biosensor screening methods as alternatives to the ferrozine assay

IF 1.9 4区 农林科学 Q3 CHEMISTRY, APPLIED
Mads Bjørlie, Rachel Irankunda, Betül Yesiltas, Ann-Dorit Moltke Sørensen, Jean-Michel Girardet, Sandrine Boschi-Müller, Charlotte Jacobsen, Laetitia Canabady-Rochelle
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Abstract

Preventing metal-catalyzed lipid oxidation in food products, which decreases nutritional value and sensory quality, is crucial in the food industry. This is typically achieved through the use of metal-chelating molecules. While the ferrozine assay is widely used to screen protein hydrolysates for metal chelating activity, it has proven difficult to use with pure peptides. This study evaluates the potential of surface plasmon resonance (SPR) and electrically switchable nanolever technology (switchSENSE®) as alternative screening methods. Unfortunately, solubility issues and large standard deviations precluded a direct correlation between the ferrozine assay and these biosensor techniques. Both techniques, however, were able to quantitatively distinguish between two peptides with very similar sequences despite the absence of a correlation between dissociation constants determined by SPR and switchSENSE®. This study highlights the potential of SPR and switchSENSE® for screening the metal chelating activity of pure peptides, advancing the understanding of peptide-metal ion interactions.

Abstract Image

金属螯合抗氧化肽:生物传感器筛选方法可替代铁嗪测定法
金属催化的脂质氧化会降低食品的营养价值和感官质量,防止这种氧化在食品工业中至关重要。这通常是通过使用金属螯合分子来实现的。虽然铁嗪试验被广泛用于筛选蛋白质水解物的金属螯合活性,但事实证明它很难用于纯肽。本研究评估了表面等离子体共振(SPR)和电可切换纳米杠杆技术(switchSENSE®)作为替代筛选方法的潜力。遗憾的是,溶解度问题和较大的标准偏差排除了铁嗪检测与这些生物传感器技术之间的直接相关性。不过,尽管 SPR 和 switchSENSE® 测定的解离常数之间缺乏相关性,但这两种技术都能定量区分序列非常相似的两种肽。这项研究凸显了 SPR 和 switchSENSE® 在筛选纯肽的金属螯合活性方面的潜力,从而加深了人们对肽与金属离子相互作用的理解。
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来源期刊
CiteScore
4.10
自引率
5.00%
发文量
95
审稿时长
2.4 months
期刊介绍: The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate. JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of year­to­year, environmental, and/ or cultivar variations through use of appropriate statistical analyses.
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