Beneficial Effects of Probiotics on Liver Injury Caused by Chronic Alcohol Consumption

Fermentation Pub Date : 2024-02-23 DOI:10.3390/fermentation10030127

Jian Sang, Hengxian Qu, Dong Liu, Yunchao Wa, Dawei Chen, Xia Chen, R. Gu, Yujun Huang

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Abstract

Alcoholic liver injury is a serious risk to human health. Probiotics have become a popular form of treatment. Lacticaseibacillus casei Grx12 and Limosilactobacillus fermentum Grx07 isolated from the gut of long-lived people in Rugao, Jiangsu, were studied to determine their protective effects and possible mechanisms of action on alcoholic liver injury. The results showed that rat serum ALT and AST were restored, and liver injury was reduced after the probiotics intervention. The level of antioxidant enzymes and antioxidants such as SOD, GSH and GSH-Px in the rat liver was significantly increased (p < 0.05), which reduces the level of MDA, a peroxidation product in the liver, and thus alleviates liver oxidative stress. L. casei Grx12 and L. fermentum Grx07 also could significantly enhance the expression of Nrf2 protein in the rat liver to regulate the anti-oxidative stress response in the body and cells (p < 0.05). The levels of ADH, Na+-K+-ATPase and Ca2+-ATPase in the rat liver were significantly increased (p < 0.05), which enhanced the body’s metabolism of alcohol. The rat serum LPS and liver TNF-α, IL-6, VEGF, TGF-β1 and NF-κB levels were significantly reduced (p < 0.05), indicating that the probiotics could relieve liver inflammation. The results of this study indicate that L. casei Grx12 and L. fermentum Grx07 have certain protective effects on alcoholic liver injury in rats, likely because of their antioxidant properties and ability to prevent oxidative stress and relieve inflammation.

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益生菌对长期饮酒导致的肝损伤的有益影响

酒精性肝损伤严重危害人类健康。益生菌已成为一种流行的治疗方式。研究人员从江苏如皋长寿人群的肠道中分离出乳酸酶杆菌Grx12和低硅乳杆菌发酵菌Grx07，以确定它们对酒精性肝损伤的保护作用和可能的作用机制。结果表明，益生菌干预后，大鼠血清谷丙转氨酶和谷草转氨酶得到恢复，肝损伤减轻。大鼠肝脏中的 SOD、GSH 和 GSH-Px 等抗氧化酶和抗氧化剂的水平显著提高（p < 0.05），从而降低了肝脏中过氧化产物 MDA 的水平，缓解了肝脏氧化应激。L. casei Grx12 和 L. fermentum Grx07 还能显著提高大鼠肝脏中 Nrf2 蛋白的表达，从而调节机体和细胞的抗氧化应激反应（p < 0.05）。大鼠肝脏中的 ADH、Na+-K+-ATPase 和 Ca2+-ATPase 水平明显提高（p < 0.05），从而增强了机体对酒精的代谢。大鼠血清 LPS 和肝脏 TNF-α、IL-6、VEGF、TGF-β1 和 NF-κB 水平明显降低（p < 0.05），说明益生菌能缓解肝脏炎症。本研究结果表明，L. casei Grx12 和 L. fermentum Grx07 对大鼠酒精性肝损伤具有一定的保护作用，这可能是因为它们具有抗氧化特性，能够防止氧化应激和缓解炎症。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Fermentation

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