Transcriptional profiling of geniposide bioconversion into genipin during gardenia fructus extract fermentation by Lactobacillus (Lactiplantibacillus) plantarum SN13T.

IF 2.5 Q3 MICROBIOLOGY
Bioscience of microbiota, food and health Pub Date : 2024-01-01 Epub Date: 2023-12-18 DOI:10.12938/bmfh.2023-066
Shrijana Shakya, Narandalai Danshiitsoodol, Masafumi Noda, Masanori Sugiyama
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引用次数: 0

Abstract

Lactiplantibacillus plantarum SN13T is a probiotic plant-derived lactic acid bacterium that can grow in various medicinal plant extracts. In this study, we fermented an aqueous extract of gardenia fructus, the fruit of a medicinal plant, with SN13T, such that the bioactivity of the extract was potentiated after fermentation to suppress the release of inflammatory mediators, such as nitric oxide (NO), reactive oxygen species (ROS), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), as well as downregulate inflammatory genes in lipopolysaccharides (LPS)-stimulated RAW 264.7 cells. This increased antioxidant and anti-inflammatory activity was mediated through bioconversion of the iridoid glycoside geniposide to its aglycone genipin via the supposed hydrolytic action of β-glucosidases harbored by SN13T. In the complete genome of SN13T, ten putative genes encoding β-glucosidases of glycosyl hydrolase (GH) family 1 organized among eight gene operons were identified. Transcriptional profiling revealed that two 6-phospho-β-glucosidase genes, pbg9 and SN13T_1925, located adjacently in the gene operon SN13T_1923, were transcribed significantly more than the remaining genes during fermentation of the gardenia extract. This suggests the role of these β-glucosidases in bioconversion of geniposide to genipin and the subsequent enhanced bioactivity of the gardenia fructus extract after fermentation with SN13T.

植物乳杆菌(Lactiplantibacillus)SN13T 在栀子果提取物发酵过程中将玄皮苷生物转化为玄皮素的转录谱分析。
植物乳杆菌(Lactiplantibacillus plantarum SN13T)是一种源于植物的益生乳酸菌,可在多种药用植物提取物中生长。在这项研究中,我们用 SN13T 发酵了一种药用植物果实栀子的水提取物,发酵后提取物的生物活性得到了增强,从而抑制了一氧化氮(NO)、活性氧(ROS)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)等炎症介质的释放,并下调了脂多糖(LPS)刺激的 RAW 264.7 细胞中的炎症基因。7 细胞中的炎症基因。这种增强的抗氧化和抗炎活性是通过 SN13T 所携带的 β-葡萄糖苷酶的水解作用,将鸢尾甙玄皮苷生物转化为其苷元玄皮素而介导的。在 SN13T 的完整基因组中,发现了 10 个编码糖基水解酶(GH)家族 1 β-糖苷酶的假定基因,这些基因由 8 个基因操作子组成。转录谱分析显示,在栀子提取物的发酵过程中,位于基因操作子 SN13T_1923 中的两个 6-磷酸-β-葡萄糖苷酶基因 pbg9 和 SN13T_1925 的转录量明显高于其余基因。这表明这些 β-葡萄糖苷酶在玄参皂苷向玄参素的生物转化过程中起着重要作用,栀子果实提取物经 SN13T 发酵后的生物活性也随之增强。
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CiteScore
6.20
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