Oxygen imaging of hypoxic pockets in the mouse cerebral cortex

IF 44.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2024-03-28 DOI:10.1126/science.adn1011

Felix R. M. Beinlich, Antonios Asiminas, Verena Untiet, Zuzanna Bojarowska, Virginia Plá, Björn Sigurdsson, Vincenzo Timmel, Lukas Gehrig, Michael H. Graber, Hajime Hirase, Maiken Nedergaard

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引用次数: 0

Abstract

Consciousness is lost within seconds upon cessation of cerebral blood flow. The brain cannot store oxygen, and interruption of oxidative phosphorylation is fatal within minutes. Yet only rudimentary knowledge exists regarding cortical partial oxygen tension (Po₂) dynamics under physiological conditions. Here we introduce Green enhanced Nano-lantern (GeNL), a genetically encoded bioluminescent oxygen indicator for Po₂ imaging. In awake behaving mice, we uncover the existence of spontaneous, spatially defined “hypoxic pockets” and demonstrate their linkage to the abrogation of local capillary flow. Exercise reduced the burden of hypoxic pockets by 52% compared with rest. The study provides insight into cortical oxygen dynamics in awake behaving animals and concurrently establishes a tool to delineate the importance of oxygen tension in physiological processes and neurological diseases.

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小鼠大脑皮层缺氧袋的氧成像。

脑血流停止后，意识会在几秒钟内丧失。大脑无法储存氧气，氧化磷酸化过程的中断会在几分钟内致命。然而，人们对生理条件下大脑皮层部分氧张力（Po2）的动态了解甚少。在这里，我们引入了绿色增强型纳米灯笼（GeNL），这是一种用于Po2成像的基因编码生物发光氧指示剂。在清醒的行为小鼠中，我们发现了自发的、空间上确定的 "缺氧口袋"，并证明了它们与局部毛细血管流量减弱之间的联系。与静止状态相比，运动使缺氧袋的负担减少了 52%。这项研究深入揭示了清醒动物大脑皮层的氧动态，同时还建立了一种工具，用于描述氧张力在生理过程和神经系统疾病中的重要性。

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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