Laboratory evaluation of a rapid diagnostic test for dairy mastitis.

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Journal of Dairy Research Pub Date : 2024-02-01 Epub Date: 2024-03-18 DOI:10.1017/S0022029924000104
David C Speksnijder, Hendrika C Verduijn, Sharon van Haren, Tomas Ussing, Tine van Werven
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Abstract

Rapid diagnostic tests that differentiate between Gram positive, Gram negative and the absence of aerobic bacteria in milk samples from dairy cows with clinical mastitis can support antimicrobial treatment decisions and contribute to a more prudent use of antimicrobials in the dairy industry. The objective of this study was to evaluate the test characteristics of the novel rapid BACT mastitis test in discriminating causes of clinical mastitis under laboratory conditions. Test outcomes of 155 milk samples from clinical mastitis cases were incubated for 14-16 h in the BACT test and compared to results of bacteriological culture. The accuracy for detection of bacterial growth and Gram positive growth was 91 and 89%, respectively. The BACT test could provide an accurate and relatively fast decision tool for farmers to aid in antimicrobial treatment decisions in cases of clinical mastitis.

奶牛乳腺炎快速诊断测试的实验室评估。
快速诊断检测可区分临床乳腺炎奶牛奶样中的革兰氏阳性菌、革兰氏阴性菌和不需氧菌,有助于做出抗菌治疗决定,并有助于乳制品行业更谨慎地使用抗菌素。本研究的目的是评估新型快速 BACT 乳腺炎检验在实验室条件下鉴别临床乳腺炎病因的检验特性。将 155 份来自临床乳腺炎病例的牛奶样本在 BACT 检验中培养 14-16 小时后,将检验结果与细菌培养结果进行比较。细菌生长和革兰氏阳性生长的检测准确率分别为 91% 和 89%。BACT 试验可为奶农提供准确、相对快速的决策工具,帮助他们在临床乳腺炎病例中做出抗菌治疗决定。
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来源期刊
Journal of Dairy Research
Journal of Dairy Research 农林科学-奶制品与动物科学
CiteScore
3.80
自引率
4.80%
发文量
117
审稿时长
12-24 weeks
期刊介绍: The Journal of Dairy Research is an international Journal of high-standing that publishes original scientific research on all aspects of the biology, wellbeing and technology of lactating animals and the foods they produce. The Journal’s ability to cover the entire dairy foods chain is a major strength. Cross-disciplinary research is particularly welcomed, as is comparative lactation research in different dairy and non-dairy species and research dealing with consumer health aspects of dairy products. Journal of Dairy Research: an international Journal of the lactation sciences.
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