Biosynthesized AgNP Modified Glassy Carbon Electrode as a Bacteria Sensor Based on Amperometry and Impedance-Based Detection

Abstract

The most effective methods for detecting bacterial cells at different phases of development take a lot of time, require expert labor, and call for state-of-the-art lab setups, including complex equipment and surroundings. Here, using amperometry and non-faradaic electrochemical impedance spectroscopy (nf-EIS) measurements, we have developed a glassy carbon electrode (GCE) derived from carbon-microelectromechanical systems (C-MEMS) that has been bio-modified to detect the impact of biologically synthesized silver nanoparticles on bacterial cells. The measurement method is more straightforward and accurate because no labeling molecules or redox markers are used. Using a standard bioassay method, the antibacterial properties of the synthesized nanoparticles were established. Amperometry and impedance readings were then used to determine when the concentration of the cells had decreased. The electroanalytical analysis was performed using the chronoamperometry method under optimal conditions. Rapid antibacterial testing at the point-of-need, a significant problem in water quality management, is made possible thanks to these findings.