Mutations in KASIIB result in increased levels of palmitic acid in soybean seeds

IF 1.9 4区农林科学 Q3 CHEMISTRY, APPLIED

Journal of the American Oil Chemists Society Pub Date : 2024-03-05 DOI:10.1002/aocs.12827

Rachel Devereaux, Militza Carrero-Colón, Karen Hudson

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Abstract

The plasticity of the lipid profile of soybean oil has made the soybean a crop with value in feed, food and industrial uses. Soybean oil typically consists of approximately 10% of the fully saturated palmitic acid, which has value for industrial purposes, however it is generally undesirable in food applications. Two soybean KASII (ketoacyl-ACP synthase II) genes have been previously implicated in the control of seed palmitic acid levels. Using forward genetics, we isolated four novel alleles in the GmKASIIB gene that result in increased levels of palmitic acid, ranging from 13% to 16% of the total fatty acid content, over multiple seasons in the field. Three of the KASIIB mutations result in independent amino acid substitutions at conserved positions in the KASII protein, and one single nucleotide polymorphism is likely to cause aberrant splicing of the KASIIB transcript. These new mutations can be used in breeding applications to increase palmitic acid content and to probe the control of fatty acid biosynthesis in soybean seeds.

New alleles of the GmKASIIb gene result in increased levels of palmitic acid in soybean seeds.

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KASIIB 基因突变导致大豆种子中棕榈酸含量增加

大豆油脂质的可塑性使大豆成为一种具有饲料、食品和工业用途价值的作物。大豆油中通常含有约 10% 的完全饱和棕榈酸，具有工业用途价值，但在食品应用中通常不受欢迎。两个大豆 KASII（酮酰基-ACP 合酶 II）基因曾被认为与控制种子棕榈酸水平有关。通过正向遗传学方法，我们在 GmKASIIB 基因中分离出了四种新型等位基因，这些等位基因在田间多季生长过程中会导致棕榈酸含量增加，占总脂肪酸含量的 13% 到 16%。其中三个 KASIIB 基因突变导致 KASII 蛋白保守位置上的独立氨基酸置换，一个单核苷酸多态性可能导致 KASIIB 转录本的剪接异常。这些新突变可用于育种，以提高棕榈酸含量，并探究大豆种子中脂肪酸生物合成的控制。

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来源期刊

Journal of the American Oil Chemists Society 工程技术-食品科技

CiteScore

4.10

自引率

5.00%

发文量

审稿时长

2.4 months

期刊介绍： The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate. JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of yeartoyear, environmental, and/ or cultivar variations through use of appropriate statistical analyses.