Unlocking the plant growth-promoting potential of yeast spp.: exploring species from the Moroccan extremophilic environment for enhanced plant growth and sustainable farming.

IF 2.2 4区 生物学 Q3 MICROBIOLOGY
Anas Raklami, Olubukola Oluranti Babalola, Martin Jemo, Ahmed Nafis
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Abstract

In this study, we successfully isolated two distinct yeasts from Moroccan extreme environments. These yeasts were subjected to molecular characterization by analyzing their Internal Transcribed spacer (ITS) regions. Our research thoroughly characterizes plant growth-promoting abilities and their drought and salt stress tolerance. In a greenhouse assay, we examined the impact of selected yeasts on Medicago sativa's growth. Four treatments were employed: (i) control without inoculation (NI), (ii) inoculation with L1, (iii) inoculation with L2, and (iv) inoculation with the mixture L1 + L2. L1 isolated from Toubkal Mountain shared 99.83% sequence similarity to Rhodotorula mucilaginosa. Meanwhile, L2, thriving in the arid Merzouga desert, displayed a similar identity to Naganishia albida (99.84%). Yeast strains were tolerant to NaCl (2 M) and 60% PEG (polyethylene glycol P6000) in case of drought. Both strains could solubilize phsphorus, with L2 additionally demonstrating potassium solubilization. In addition, both strains produce indole acetic acid (up to 135 µl ml-1), have siderophore ability, and produce aminocyclopropane-1-carboxylic acid deaminase. Isolates L1 and L2, and their consortium showed that the single or combined strain inoculation of M. sativa improved plant growth, development, and nutrient assimilation. These findings pave the way for harnessing yeast-based solutions in agricultural practices, contributing to enhanced crop productivity and environmental sustainability.

发掘酵母菌对植物生长的促进潜力:探索摩洛哥嗜极环境中的物种,促进植物生长和可持续农业。
在这项研究中,我们成功地从摩洛哥极端环境中分离出两种不同的酵母菌。通过分析它们的 ITS 区域,对这些酵母菌进行了分子鉴定。我们的研究深入探讨了植物生长促进(PGP)能力及其对干旱和盐胁迫的耐受性。在温室试验中,我们考察了所选酵母菌对美智子生长的影响。我们采用了四种处理方法:i)不接种对照(NI);ii)接种 L1;iii)接种 L2;iv)接种 L1+L2 混合物。从 Toubkal 山分离出的 L1 与 Rhodotorula mucilaginosa 的序列相似度为 99.83%。同时,在干旱的梅尔祖加沙漠中生长的 L2 与 Naganishia albida(99.84%)具有相似性。酵母菌株对 2 M 的 NaCl 和干旱情况下 60% 的 PEG(聚乙二醇 P6000)具有耐受性。两株菌株都能溶解 P 和 K(以 L2 为例),产生 IAA(高达 135 µl mL-1),具有苷元能力,并能产生 ACC 脱氨酶。分离菌株 L1、L2 及其联合菌株表明,将单一或联合菌株接种到荠属植物上可改善植物的生长、发育和养分吸收。这些发现为在农业实践中利用基于酵母的解决方案铺平了道路,有助于提高作物产量和环境可持续性。
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来源期刊
Fems Microbiology Letters
Fems Microbiology Letters 生物-微生物学
CiteScore
4.30
自引率
0.00%
发文量
112
审稿时长
1.9 months
期刊介绍: FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.
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