Enhancement of yellow pigments production via high CaCl2 stress fermentation of Monascus purpureus.

IF 2.2 4区生物学 Q3 MICROBIOLOGY

Fems Microbiology Letters Pub Date : 2024-01-09 DOI:10.1093/femsle/fnae012

Yuqing Bai, Wei Zhang, Ruonan Guo, Jiyuan Yu, Yurong Wang

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Abstract

Monascus pigments (MPs) are a kind of natural ingredient fermented by Monascus spp., which contains three types of pigments: red, orange, and yellow ones. Monascus yellow pigments have a restricted yield and cannot meet industrial application. The method and mechanism of CaCl2 improving yellow pigments production by liquid fermentation of Monascus purpureus M8 were studied in order to overcome the low yield of yellow pigments produced by liquid fermentation. Changes in physiological and biochemical indicators explained the effects of CaCl2 on the production of Monascus yellow pigments from solid fermentation. The intracellular yellow pigments, orange pigments, and red pigments increased by 156.08%, 43.76%, and 42.73%, respectively, with 60 g/l CaCl2 addition to culture medium. The amount of red and orange pigments reduced, while the proportion of yellow pigments increased and the relative peak area of intracellular yellow pigments accounted for a dominant 98.2%, according to thin layer chromatography and high performance liquid chromatography analyses. Furthermore, the influence of CaCl2 extended to the modulation of pigments synthesis-related gene expression in M8 strain. This modulation led to a pronounced upregulation in the expression of the yellow pigments synthesis-related gene, mppE, signifying a pivotal role played by CaCl2 in orchestrating the intricate machinery behind yellow pigments biosynthesis.

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通过高 CaCl2 胁迫发酵紫云英提高黄色素产量

莫纳斯卡色素（MPs）是一种由莫纳斯卡属植物发酵而成的天然成分，含有红色、橙色和黄色三种色素。莫纳斯卡黄色素的产量有限，无法满足工业应用。为了克服液体发酵生产黄色素产量低的问题，研究了 CaCl2 提高 M. purpureus M8 液体发酵生产黄色素的方法和机理。生理生化指标的变化解释了 CaCl2 对固体发酵生产 Monascus 黄色素的影响。在培养基中添加 60 g/L CaCl2 后，细胞内黄色素、橙色素和红色素分别增加了 156.08%、43.76% 和 42.73%。根据薄层色谱和高效液相色谱分析，红色素和橙色素的含量减少，而黄色素的比例增加，细胞内黄色素的相对峰面积占 98.2%。此外，CaCl2 对 M8 菌株中色素合成相关基因的表达也有影响。这种调控导致黄色素合成相关基因 mppE 的表达明显上调，表明 CaCl2 在协调黄色素生物合成背后的复杂机制中发挥了关键作用。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.