Evaluation of Dietary Essential Amino Acid Supplementation on Growth, Digestive Capacity, Antioxidant, and Intestine Health of the Juvenile Redclaw Crayfish, Cherax quadricarinatus

IF 3 2区农林科学 Q1 FISHERIES

Aquaculture Nutrition Pub Date : 2024-02-08 DOI:10.1155/2024/8767751

Zongzheng Jiang, Dunwei Qian, Zhenye Liang, Sen Wu, Fenglu Han, Chang Xu, Meili Chi, Erchao Li

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引用次数: 0

Abstract

The present study was an 8-week feeding trial investigating the effects of lysine and threonine supplementation in vegetable-based diets on growth, antioxidative capacity, and gut microbiota of juvenile redclaw crayfish, Cherax quadricarinatus (initial weight 11.52 ± 0.23 g). The lysine and threonine were supplemented to formulate five isonitrogenous (37%) and isolipidic (9%) diets containing 0% (control), 0.2% lysine (L0.2), 0.2% threonine (T0.2), 0.4% lysine (L0.4), and 0.4% threonine (T0.4), respectively. Compared to the control, weight gain rate (WGR) and specific growth rate (SGR) of C. quadricarinatus significantly increased with increasing dietary lysine and threonine supplementation from 0.2% to 0.4% (P < 0.05). Hepatopancreas trypsin activity significantly increased with increasing levels of lysine and threonine in diets (P < 0.05). However, the pepsin, lipase, and amylase activities were not affected by dietary levels of lysine and threonine (P > 0.05). Compared with the control, crayfish in T0.4 and L0.4 showed significantly higher glutathione peroxidase (GPx) activity (P < 0.05), lower alanine aminotransferase (ALT) activity, and lower malondialdehyde (MDA) content (P < 0.05). Supplementation with 0.4% lysine significantly changed the composition of the gut microbiota (P < 0.05), which showed a significantly increased relative abundance of Proteobacteria and decreased Firmicutes, Actinomycetes, and Pontomyces (P < 0.05). The PICRUSt analysis demonstrated that the abundance of the metabolism and cellular processes pathways in the L0.4 group were markedly decreased compared with the control (P < 0.05). Meanwhile, a tighter interaction of the microbiota community in crayfish was observed in the T0.4 experimental group. In conclusion, these results suggested that dietary supplementation with 0.4% threonine could significantly promote growth and improve microbial health in juvenile C. quadricarinatus.

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补充膳食必需氨基酸对红爪螯虾幼鱼（Cherax quadricarinatus）生长、消化能力、抗氧化性和肠道健康的评估

本研究是一项为期 8 周的饲养试验，调查在以蔬菜为基础的日粮中添加赖氨酸和苏氨酸对红爪螯虾（Cherax quadricarinatus，初始体重为 11.52 ± 0.23 克）幼体的生长、抗氧化能力和肠道微生物群的影响。通过补充赖氨酸和苏氨酸，配制出五种等氮型（37%）和离脂型（9%）日粮，分别含有0%（对照组）、0.2%赖氨酸（L0.2）、0.2%苏氨酸（T0.2）、0.4%赖氨酸（L0.4）和0.4%苏氨酸（T0.4）。与对照组相比，随着日粮中赖氨酸和苏氨酸添加量从 0.2% 增加到 0.4%，C. quadricarinatus 的增重率（WGR）和特定生长率（SGR）显著增加（P < 0.05）。随着日粮中赖氨酸和苏氨酸水平的增加，肝胰脏胰蛋白酶活性也明显增加（P < 0.05）。然而，胃蛋白酶、脂肪酶和淀粉酶活性不受日粮中赖氨酸和苏氨酸水平的影响（P > 0.05）。与对照组相比，T0.4 和 L0.4 小龙虾的谷胱甘肽过氧化物酶（GPx）活性明显提高（P < 0.05），丙氨酸氨基转移酶（ALT）活性降低，丙二醛（MDA）含量降低（P < 0.05）。补充 0.4% 的赖氨酸后，肠道微生物群的组成发生了显著变化（P < 0.05），其中蛋白质细菌的相对丰度显著增加，而真菌、放线菌和庞特真菌的相对丰度则有所下降（P < 0.05）。PICRUSt 分析表明，与对照组相比，L0.4 组代谢和细胞过程途径的丰度明显下降（P < 0.05）。同时，T0.4 实验组中小龙虾微生物群落的相互作用更加紧密。总之，这些结果表明，膳食中补充 0.4% 苏氨酸可显著促进四大家鱼幼体的生长并改善微生物健康。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture Nutrition 农林科学-渔业

CiteScore

7.20

自引率

8.60%

发文量

131

审稿时长

3 months

期刊介绍： Aquaculture Nutrition is published on a bimonthly basis, providing a global perspective on the nutrition of all cultivated aquatic animals. Topics range from extensive aquaculture to laboratory studies of nutritional biochemistry and physiology. The Journal specifically seeks to improve our understanding of the nutrition of aquacultured species through the provision of an international forum for the presentation of reviews and original research papers. Aquaculture Nutrition publishes papers which strive to: increase basic knowledge of the nutrition of aquacultured species and elevate the standards of published aquaculture nutrition research. improve understanding of the relationships between nutrition and the environmental impact of aquaculture. increase understanding of the relationships between nutrition and processing, product quality, and the consumer. help aquaculturalists improve their management and understanding of the complex discipline of nutrition. help the aquaculture feed industry by providing a focus for relevant information, techniques, tools and concepts.