The MAT1 locus is required for microconidia-mediated sexual fertility in the rice blast fungus.

IF 2.2 4区生物学 Q3 MICROBIOLOGY

Fems Microbiology Letters Pub Date : 2024-01-09 DOI:10.1093/femsle/fnae004

Kohtetsu Kita, Momotaka Uchida, Tsutomu Arie, Tohru Teraoka, Hisatoshi Kaku, Yasukazu Kanda, Masaki Mori, Takayuki Arazoe, Takashi Kamakura

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引用次数: 0

Abstract

Rice blast fungus (Pyricularia oryzae) is a heterothallic ascomycete that causes the most destructive disease in cultivated rice worldwide. This fungus reproduces sexually and asexually, and its mating type is determined by the MAT1 locus, MAT1-1 or MAT1-2. Interestingly, most rice-infecting field isolates show a loss of female fertility, but the MAT1 locus is highly conserved in female-sterile isolates. In this study, we performed a functional analysis of MAT1 using the CRISPR/Cas9 system in female- and male-fertile isolates and female-sterile (male-fertile) isolates. Consistent with a previous report, MAT1 was essential for sexual reproduction but not for asexual reproduction. Meanwhile, deletion mutants of MAT1-1-1, MAT1-1-2, and MAT1-1-3 exhibited phenotypes different from those of other previously described isolates, suggesting that the function of MAT1-1 genes and/or their target genes in sexual reproduction differs among strains or isolates. The MAT1 genes, excluding MAT1-2-6, retained their functions even in female-sterile isolates, and deletion mutants lead to loss or reduction of male fertility. Although MAT1 deletion did not affect microconidia (spermatia) production, microconidia derived from the mutants could not induce perithecia formation. These results indicated that MAT1 is required for microconidia-mediated male fertility in addition to female fertility in P. oryzae .

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MAT1 基因座是水稻稻瘟病菌由小孢子介导的有性繁殖力所必需的。

稻瘟病真菌（Pyricularia oryzae）是一种异型子囊菌，是全球水稻栽培中最具毁灭性的病害。这种真菌有性生殖和无性生殖，其交配类型由 MAT1 基因座（MAT1-1 或 MAT1-2）决定。有趣的是，大多数感染水稻的田间分离株都会丧失雌性繁殖力，但在雌性不育的分离株中，MAT1 基因座却高度保守。在本研究中，我们利用 CRISPR/Cas9 系统对雌性和雄性不育分离株以及雌性不育（雄性不育）分离株中的 MAT1 进行了功能分析。与之前的报告一致，MAT1 对有性生殖至关重要，但对无性生殖却不重要。同时，MAT1-1-1、MAT1-1-2 和 MAT1-1-3 的缺失突变体表现出的表型与之前描述的其他分离株不同，这表明不同菌株或分离株的 MAT1-1 基因和/或其靶基因在有性生殖中的功能不同。MAT1 基因（不包括 MAT1-2-6）即使在雌性不育分离株中也能保留其功能，缺失突变体会导致雄性繁殖力丧失或降低。虽然 MAT1 基因缺失并不影响微孢子囊（精子）的产生，但突变体产生的微孢子囊不能诱导包囊的形成。这些结果表明，除了雌性繁殖力之外，MAT1 也是 P. oryzae 中微囊介导的雄性繁殖力所必需的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.