Simulated gastrointestinal digestion of walnut protein yields anti-inflammatory peptides

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2024-02-03 DOI:10.1016/j.foodchem.2024.138646

Wei Xia , Yuan Gao , Xiangjun Fang , Long Jin , Ruiling Liu , Li-Shu Wang , Yangyong Deng , Junlong Gao , Hailong Yang , Weijie Wu , Haiyan Gao

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Abstract

The impact of the simulated gastrointestinal digestion process on walnut protein and the potential anti-inflammatory properties of its metabolites was studied. Structural changes induced by digestion, notably in α-Helix, β-Turn, and Random Coil configurations, were unveiled. Proteins over 10,000 Da significantly decreased by 35.6 %. Antioxidant activity in these metabolites paralleled increased amino acid content. Molecular docking identified three walnut polypeptides—IPAGTPVYLINR, FQGQLPR, and VVYVLR—with potent anti-inflammatory properties. RMSD and RMSF analysis demonstrated the stable and flexible interaction of these polypeptides with their target proteins. In lipopolysaccharide (LPS)-induced inflammation in normal human colon mucosal epithelial NCM460 cells, these peptides decreased 5-hydroxytryptamine (5-HT), tumor necrosis factor-alpha (TNF-α), and vascular endothelial growth factor (VEGF) expression, while mitigating cell apoptosis and inflammation. Our study offers valuable insights into walnut protein physiology, shedding light on its potential health benefits.

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核桃蛋白的模拟胃肠道消化产生抗炎肽

研究了模拟胃肠道消化过程对核桃蛋白质的影响及其代谢物的潜在抗炎特性。研究揭示了消化引起的结构变化，特别是 α-elix、β-Turn 和随机线圈构型的变化。超过 10,000 Da 的蛋白质明显减少了 35.6%。这些代谢物的抗氧化活性与氨基酸含量的增加相一致。分子对接发现了三种核桃多肽--IPAGTPVYLINR、FQGQLPR 和 VVYVLR--具有强大的抗炎特性。RMSD 和 RMSF 分析表明了这些多肽与其目标蛋白质之间稳定而灵活的相互作用。在脂多糖（LPS）诱导的正常人结肠粘膜上皮 NCM460 细胞炎症中，这些多肽降低了 5-羟色胺（5-HT）、肿瘤坏死因子-α（TNF-α）和血管内皮生长因子（VEGF）的表达，同时减轻了细胞凋亡和炎症。我们的研究为核桃蛋白生理学提供了宝贵的见解，揭示了其潜在的健康益处。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.