Multilocus genotyping of sugarcane white leaf phytoplasma in Thailand

IF 2.5 3区农林科学 Q2 Agricultural and Biological Sciences

Tropical Plant Pathology Pub Date : 2024-01-29 DOI:10.1007/s40858-024-00635-7

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引用次数: 0

Abstract

One hundred and seventy-four symptomatic sugarcane samples infected with sugarcane white leaf (SCWL) phytoplasma were collected from sugarcane growing areas in ten provinces across the North, Northeastern and Central plain of Thailand. Genotyping was performed using 16S rRNA and ITS sequence and multilocus sequence typing (MLST) based on six genes including tuf, secY, leuS, secA, AAA1 and groES. The 16S rRNA sequence analysis indicated that the SCWL phytoplasma population showed nucleotide sequence identity of 99.74% to 99.80% to ‘Candidatus Phytoplasma sacchari’. In silico RFLP analysis clustered the strains into 16SrXI-B and 16SrXI-D subgroups. Most strains (77%) were in the 16SrXI-D group, while 16SrXI-B strains were only found in the Northeastern regions. The 16SrXI-D group coexisted with the 16SrXI-B group in Roi Et, Kalasin, and Mookdahan provinces, but it was found alone in Surin province. Multilocal sequence typing showed genetic variations in six genes including tuf, secY, leuS, secA, AAA1, and groES. These variations resulted in the SCWL population being divided into two distinct groups, that are consistent with the 16SrXI subgroups. Additionally, five strains in this population exhibited recombinant DNA in the tuf, secA, leuS, AAA1 and groES genes. This information about the genetic variability of SCWL phytoplasma in Thailand could be used to develop detection methods and monitoring strategies for the disease management.

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泰国甘蔗白叶植物支原体的多焦点基因分型

摘要从泰国北部、东北部和中部平原 10 个府的甘蔗种植区采集了 174 份感染甘蔗白叶（SCWL）植原体的有症状甘蔗样本。利用 16S rRNA 和 ITS 序列以及基于六个基因（包括 tuf、secY、leuS、secA、AAA1 和 groES）的多焦点序列分型（MLST）进行了基因分型。16S rRNA序列分析表明，SCWL植物支原体种群与 "Candidatus Phytoplasma sacchari "的核苷酸序列同一性为99.74%至99.80%。默观 RFLP 分析将菌株分为 16SrXI-B 和 16SrXI-D 亚群。大多数菌株（77%）属于 16SrXI-D 组，而 16SrXI-B 菌株只出现在东北地区。16SrXI-D组与16SrXI-B组在罗易府、加拉信府和莫克达罕府共存，但在素林府却单独发现。多定位序列分型显示了六个基因的遗传变异，包括 tuf、secY、leuS、secA、AAA1 和 groES。这些变异导致 SCWL 群体被分为两个不同的群体，与 16SrXI 亚群一致。此外，该群体中有五个菌株在 tuf、secA、leuS、AAA1 和 groES 基因中表现出 DNA 重组。这些有关泰国 SCWL 植原体遗传变异的信息可用于开发检测方法和制定病害管理监测策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tropical Plant Pathology PLANT SCIENCES-

CiteScore

4.50

自引率

4.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.  Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection. The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines. Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.