Interfacial and emulsion properties of albumin-rich fractions from sunflower seeds

IF 1.9 4区 农林科学 Q3 CHEMISTRY, APPLIED
Sabrina Diekmann, Stephan Drusch, Monika Brückner-Gühmann
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引用次数: 0

Abstract

The interfacial and emulsion properties of mixed albumin-rich fractions extracted from sunflower seeds at pH 3 and pH 5 were analyzed. SDS-PAGE under reducing and non-reducing conditions was used to visualize differences between the protein fractions. The interfacial activity of the albumin-rich fractions was analyzed via drop contour measurement. The viscoelasticity of the protein film the interactions between the protein molecules, and the network forming within the protein film were measured by interfacial shear rheology. Besides being both surface active, albumin-rich fractions extracted at pH 5 were found to form interfacial films that exhibited a greater stability against deformation. In consequence, emulsions prepared with pH 5 extracts showed better properties represented by a smaller oil droplet size and a lower creaming index. The results proof that mixed albumin-rich fractions can stabilize emulsions. Moreover, the presence of co-extracted phenolic compounds seems to be important to generate systems with increased elastic properties of the interfacial film.

葵花籽中富含白蛋白的馏分的界面和乳化特性
分析了在 pH 3 和 pH 5 条件下从葵花籽中提取的富含白蛋白的混合馏分的界面特性和乳化特性。在还原和非还原条件下使用 SDS-PAGE 来观察蛋白质馏分之间的差异。通过液滴轮廓测量分析了富含白蛋白的馏分的界面活性。通过界面剪切流变学测量了蛋白质膜的粘弹性、蛋白质分子之间的相互作用以及蛋白质膜内部形成的网络。结果发现,在 pH 值为 5 时提取的富含白蛋白的馏分不仅具有表面活性,而且所形成的界面薄膜具有更强的抗变形稳定性。因此,用 pH 值为 5 的萃取物制备的乳液具有更好的特性,表现为油滴尺寸更小,起皱指数更低。结果证明,富含白蛋白的混合馏分可以稳定乳液。此外,共萃取酚类化合物的存在似乎对产生具有更高的界面膜弹性特性的体系非常重要。
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来源期刊
CiteScore
4.10
自引率
5.00%
发文量
95
审稿时长
2.4 months
期刊介绍: The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate. JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of year­to­year, environmental, and/ or cultivar variations through use of appropriate statistical analyses.
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