Antimicrobial susceptibility and genotypic characterization of Escherichia coli isolated from foods controlled by the National Food Safety Agency in Burkina Faso

IF 1.4 3区农林科学 Q4 FOOD SCIENCE & TECHNOLOGY

Journal of Consumer Protection and Food Safety Pub Date : 2024-01-27 DOI:10.1007/s00003-024-01493-w

Adama Patrice Soubeiga, Dissinviel Stéphane Kpoda, Adama Sawadogo, Sibiri Sylvain Rouamba, Muller K. A. Compaoré, Asseto Belemlougri Somda, Sandrine Ouedraogo, Roukiatou Traoré, Désiré Nezien, Fulbert Nikiéma, Elie Kabre, Cheikna Zongo, Aly Savadogo

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引用次数: 0

Abstract

This study was conducted to determine the antimicrobial resistance profiles of Escherichia coli isolated from food samples received at the National Food Safety Agency in Burkina Faso. A total of 155 isolates from dairy foods (29), fish-based dishes (7), mango juices (4), lettuces (35), RTE salads (21), sandwiches (32), and sesames (27) were included for testing with the Kirby-Bauer disk diffusion method. PCR targeting ampicillin (bla_TEM, bla_SHV, temA, and temB), tetracyclines [tet(A) and tet(B)], sulfamethoxazole (sul1 and sul2), aminoglycosides (StrA and aadA) and quinolones (GyrA) resistance genes were performed to elucidate the genotypic resistance mechanism. Of the 155 isolates, 105 (67.7%) were resistant to at least one antimicrobial agent. Resistances to tetracycline (33.5%), ampicillin (32.9%), cefoxitin (18.7%), gentamycin (15.5%), amoxicillin-clavulanate acid (15.5%), nalidixic acid (12.9%), chloramphenicol (11.6%), trimethoprim-sulfamethoxazole (11.6%), and ciprofloxacin (8.4%) were observed. Multidrug resistance was recorded in 26.5% of the isolates. Antimicrobial resistance genes including bla_TEM (19/51, 37.3%), bla_SHV (19/51, 37.3%), temB (17/51, 33.3%), tet(A) (24/52, 46.2%), tet(B) (9/52, 17.3%), sul1 (8/18, 44.4%), sul2 (4/18, 22.2%), aadA (11/24, 45.83%) and GyrA (31/36, 86.1%) were detected. All E. coli isolates resistant to at least 2 antibiotics were positive for the class 1 integron gene (intI1). These findings raise concerns about food safety and public health and demonstrate the need for strict government control and continuous monitoring.

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从布基纳法索国家食品安全局控制的食品中分离的大肠埃希菌的抗菌药敏感性和基因型特征

这项研究旨在确定从布基纳法索国家食品安全局收到的食品样本中分离出的大肠埃希菌的抗菌谱。研究采用柯比-鲍尔磁盘扩散法对从乳制品（29 份）、鱼类菜肴（7 份）、芒果汁（4 份）、生菜（35 份）、即食沙拉（21 份）、三明治（32 份）和芝麻（27 份）中分离的 155 份样本进行了检测。对氨苄西林（blaTEM、blaSHV、temA 和 temB）、四环素（tet(A) 和 tet(B)）、磺胺甲噁唑（sul1 和 sul2）、氨基糖苷类（StrA 和 aadA）和喹诺酮类（GyrA）耐药基因进行 PCR 扩增，以阐明基因型耐药机制。在 155 个分离株中，有 105 个（67.7%）至少对一种抗菌药产生耐药性。对四环素（33.5%）、氨苄西林（32.9%）、头孢西丁（18.7%）、庆大霉素（15.5%）、阿莫西林-克拉维酸（15.5%）、萘啶酸（12.9%）、氯霉素（11.6%）、三甲双胍-磺胺甲噁唑（11.6%）和环丙沙星（8.4%）产生耐药性。26.5%的分离菌株对多种药物产生耐药性。检测到的耐药基因包括 blaTEM（19/51，37.3%）、blaSHV（19/51，37.3%）、temB（17/51，33.3%）、tet(A)（24/52，46.2%）、tet(B)（9/52，17.3%）、sul1（8/18，44.4%）、sul2（4/18，22.2%）、aadA（11/24，45.83%）和 GyrA（31/36，86.1%）。所有对至少两种抗生素耐药的大肠杆菌分离物的 1 类整合子基因（intI1）均呈阳性。这些发现引起了人们对食品安全和公众健康的关注，表明政府有必要进行严格控制和持续监测。

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来源期刊

Journal of Consumer Protection and Food Safety Veterinary-Food Animals

CiteScore

3.70

自引率

4.20%

发文量

审稿时长

>12 weeks

期刊介绍： The JCF publishes peer-reviewed original Research Articles and Opinions that are of direct importance to Food and Feed Safety. This includes Food Packaging, Consumer Products as well as Plant Protection Products, Food Microbiology, Veterinary Drugs, Animal Welfare and Genetic Engineering. All peer-reviewed articles that are published should be devoted to improve Consumer Health Protection. Reviews and discussions are welcomed that address legal and/or regulatory decisions with respect to risk assessment and management of Food and Feed Safety issues on a scientific basis. It addresses an international readership of scientists, risk assessors and managers, and other professionals active in the field of Food and Feed Safety and Consumer Health Protection. Manuscripts – preferably written in English but also in German – are published as Research Articles, Reviews, Methods and Short Communications and should cover aspects including, but not limited to: · Factors influencing Food and Feed Safety · Factors influencing Consumer Health Protection · Factors influencing Consumer Behavior · Exposure science related to Risk Assessment and Risk Management · Regulatory aspects related to Food and Feed Safety, Food Packaging, Consumer Products, Plant Protection Products, Food Microbiology, Veterinary Drugs, Animal Welfare and Genetic Engineering · Analytical methods and method validation related to food control and food processing. The JCF also presents important News, as well as Announcements and Reports about administrative surveillance.