[Methodologic recommendations for the determination of 25-OH vitamin D3 in plasma using HPLC].

Die Nahrung Pub Date : 1989-01-01
M Matthey, H Graf, G H Richter, G Flachowsky
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Abstract

A method for measuring 25-OH D3-vitamin (calcidiol) in plasma samples of cattle is described. The samples were purified using non-polar C 18-cartridges and 25-OH D3 was determined by high-performance liquid chromatography (HPLC) on a silicic acid straight phase liquid chromatography column (retention time 11.5 min). The detection limit for 25-OH D3 is about 0.5 ng injection quantity, the average recovery was 90%, the standard error amounted to 8%.

[HPLC法测定血浆中25-OH维生素D3的方法学建议]。
介绍了一种测定牛血浆样品中25-OH d3 -维生素(钙二醇)的方法。样品采用非极性c18柱纯化,25-OH D3采用高效液相色谱(HPLC)测定,色谱柱为硅酸直相色谱柱(保留时间11.5 min)。25-OH D3的检出限为0.5 ng左右,平均回收率为90%,标准误差为8%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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