The inhibitory effect of cisplatin combined with pemetrexed on lung cancer cells via targeting miR-135 and ATG7

IF 0.7 4区材料科学 Q3 Materials Science

Materials Express Pub Date : 2024-01-01 DOI:10.1166/mex.2024.2588

Dong Xie, Gang Zhou, Wei Zhang, Y. Gao, Xing Cai, Yang Cai, Zhiyun Liu, Weijun Zhao

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引用次数: 0

Abstract

Lung cancer treatment is still based on chemotherapy. Autophagy involves in lung cancer. Our research aims to explore miR-135’s role in lung cancer. Tumor tissues were collected for analysis of autophagy. TargetScan bioinformatics assessed the relationship between miR-135 and ATG7. Immunofluorescence analyzed the co-localization of circRACGAP1 and miR-135. circRACGAP1 was silenced to assess its role in autophagy and lung cancer cell growth. In A549 cells, cisplatin combined with pemetrexed upregulated ATG7 and LC3-II, and downregulated squestosome 1 (SQSTM1). Significantly upregulated LC3-II and ATG7 and reduced SQSTM1 were found in cisplatin combined with pemetrexed group. miR-135 targeted ATG7 gene 3′-UTR region. Cisplatin combined with pemetrexed upregulated ATG7 by selectively inhibiting miR-135. circRACGAP1 was co-localized with miR-135. circRACGAP1 inhibition decreased lung cancer cell growth by inhibiting autophagy. circRACGAP1-miR-135-ATG7 axis involves in the regulatory effect of cisplatin combined with pemetrexed on lung cancer cell growth.

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顺铂联合培美曲塞通过靶向 miR-135 和 ATG7 对肺癌细胞的抑制作用

肺癌治疗仍以化疗为主。自噬作用与肺癌有关。我们的研究旨在探讨 miR-135 在肺癌中的作用。我们收集了肿瘤组织，用于分析自噬作用。TargetScan 生物信息学评估了 miR-135 与 ATG7 的关系。免疫荧光分析了 circRACGAP1 和 miR-135 的共定位。沉默 circRACGAP1 以评估其在自噬和肺癌细胞生长中的作用。在 A549 细胞中，顺铂联合培美曲塞可上调 ATG7 和 LC3-II，下调鳞状细胞组 1 (SQSTM1)。在顺铂联合培美曲塞组中，LC3-II和ATG7显著上调，SQSTM1下调，miR-135靶向ATG7基因3′-UTR区域。circRACGAP1-miR-135-ATG7轴参与顺铂联合培美曲塞对肺癌细胞生长的调控作用。

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来源期刊

Materials Express NANOSCIENCE & NANOTECHNOLOGY-MATERIALS SCIENCE, MULTIDISCIPLINARY

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>12 weeks

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