Physiological, biochemical, and antioxidant responses of oregano subspecies (Origanum vulgare L. ssp. gracile and ssp. vulgare) to NaCl stress

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Zahra Azimzadeh, Abbas Hassani, Babak Abdollahi Mandoulakani, Ebrahim Sepehr
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引用次数: 0

Abstract

Salinity is an outstanding barrier against the production of agricultural crops, especially in arid and semi-arid regions. Oregano (Origanum vulgare L.), a valuable herb of the Lamiaceae family, contains various types of biologically active constituents such as essential oils, tannins, resins, sterols, flavonoids, and phenolic glycosides. The present research was carried out to investigate the influence of salinity stress on some physiological and biochemical attributes and antioxidant responses in two oregano subspecies (ssp. vulgare and ssp. gracile). Salt treatments were applied using irrigation with different sodium chloride concentrations (0, 25, 50, and 100 mM NaCl). The results revealed a remarkable decline in relative water content (RWC) and photosynthetic pigments in both subspecies under NaCl stress. Total soluble sugars (TSS) decreased in plants exposed to severe salt stress (100 mM NaCl), whereas H2O2 production, electrolyte leakage (EL), malondialdehyde (MDA), and leaf proline contents increased in these plants compared to control plants. A positive relationship was found between H2O2 production with EL and MDA. Furthermore, salinity improved phenolic content, antioxidant capacity, and activity of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) enzymes. The highest total flavonoid content (TFC) was achieved at 50 mM NaCl salinity, which increased by 19.33% compared to control plants. A positive relationship between the activity of phenylalanine ammonia-lyase (PAL) and TPC and TFC was observed. Analysis of phenolic compounds by HPLC showed that the amounts of gallic acid, caffeic acid, chlorogenic acid, and quercetin in ssp. gracile and caffeic acid, cinnamic acid, and quercetin in ssp. vulgare significantly increased with increasing salinity stress. In general, the findings of this study demonstrated that oregano subspecies ameliorate salt-induced osmotic and oxidative damages through increasing proline accumulation, antioxidant enzymes activity, and secondary metabolites production.

Abstract Image

Abstract Image

牛至亚种(Origanum vulgare L. ssp.
盐碱是农作物生产的一个突出障碍,尤其是在干旱和半干旱地区。牛至(Origanum vulgare L.)是一种珍贵的唇形科草本植物,含有多种生物活性成分,如精油、单宁、树脂、甾醇、黄酮类化合物和酚苷。本研究旨在调查盐胁迫对两个牛至亚种(ssp. vulgare 和 ssp. gracile)的一些生理生化属性和抗氧化反应的影响。采用不同氯化钠浓度(0、25、50 和 100 mM NaCl)的灌溉进行盐处理。结果表明,在 NaCl 胁迫下,两个亚种的相对含水量(RWC)和光合色素都明显下降。与对照植物相比,受到严重盐胁迫(100 mM NaCl)的植物总可溶性糖(TSS)减少,而 H2O2 生成量、电解质渗漏(EL)、丙二醛(MDA)和叶片脯氨酸含量增加。研究发现,H2O2 的产生与 EL 和 MDA 呈正相关。此外,盐度提高了酚含量、抗氧化能力以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)的活性。盐度为 50 mM NaCl 时,总黄酮含量(TFC)最高,比对照植物增加了 19.33%。苯丙氨酸氨解酶(PAL)活性与总黄酮和总黄酮含量之间呈正相关。通过高效液相色谱法对酚类化合物的分析表明,没食子酸、咖啡酸、绿原酸和槲皮素在禾本科植物中的含量,以及咖啡酸、肉桂酸和槲皮素在禾本科植物中的含量随着盐度胁迫的增加而显著增加。总之,本研究的结果表明,牛至亚种通过增加脯氨酸积累、抗氧化酶活性和次生代谢产物的产生来改善盐引起的渗透和氧化损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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