Carotenoid Cleavage Dioxygenase 1 and Its Application for the Production of C13-Apocarotenoids in Microbial Cell Factories: A Review

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2023-12-04 DOI:10.1021/acs.jafc.3c06459

Zhipeng Qi, Xinyi Tong, Yangyang Zhang, Shutong Jia, Xianying Fang* and Linguo Zhao*,

{"title":"Carotenoid Cleavage Dioxygenase 1 and Its Application for the Production of C13-Apocarotenoids in Microbial Cell Factories: A Review","authors":"Zhipeng Qi, Xinyi Tong, Yangyang Zhang, Shutong Jia, Xianying Fang* and Linguo Zhao*, ","doi":"10.1021/acs.jafc.3c06459","DOIUrl":null,"url":null,"abstract":"<p >C13-apocarotenoids are naturally derived from the C9–C10 (C9′–C10′) double-bond cleavage of carotenoids by carotenoid cleavage dioxygenases (CCDs). As high-value flavors and fragrances in the food and cosmetic industries, the sustainable production of C13-apocarotenoids is emerging in microbial cell factories by the carotenoid cleavage dioxygenase 1 (CCD1) subfamily. However, the commercialization of microbial-based C13-apocarotenoids is still limited by the poor performance of CCD1, which severely constrains its conversion efficiency from precursor carotenoids. This review focuses on the classification of CCDs and their cleavage modes for carotenoids to generate corresponding apocarotenoids. We then emphatically discuss the advances for C13-apocarotenoid biosynthesis in microbial cell factories with various strategies, including optimization of CCD1 expression, improvement of CCD1’s catalytic activity and substrate specificity, strengthening of substrate channeling, and development of oleaginous microbial hosts, which have been verified to increase the conversion rate from carotenoids. Lastly, the current challenges and future directions will be discussed to enhance CCDs’ application for C13-apocarotenoids biomanufacturing.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"71 49","pages":"19240–19254"},"PeriodicalIF":6.2000,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Agricultural and Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.jafc.3c06459","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

C13-apocarotenoids are naturally derived from the C9–C10 (C9′–C10′) double-bond cleavage of carotenoids by carotenoid cleavage dioxygenases (CCDs). As high-value flavors and fragrances in the food and cosmetic industries, the sustainable production of C13-apocarotenoids is emerging in microbial cell factories by the carotenoid cleavage dioxygenase 1 (CCD1) subfamily. However, the commercialization of microbial-based C13-apocarotenoids is still limited by the poor performance of CCD1, which severely constrains its conversion efficiency from precursor carotenoids. This review focuses on the classification of CCDs and their cleavage modes for carotenoids to generate corresponding apocarotenoids. We then emphatically discuss the advances for C13-apocarotenoid biosynthesis in microbial cell factories with various strategies, including optimization of CCD1 expression, improvement of CCD1’s catalytic activity and substrate specificity, strengthening of substrate channeling, and development of oleaginous microbial hosts, which have been verified to increase the conversion rate from carotenoids. Lastly, the current challenges and future directions will be discussed to enhance CCDs’ application for C13-apocarotenoids biomanufacturing.

Abstract Image

查看原文本刊更多论文

类胡萝卜素裂解双加氧酶1及其在微生物细胞工厂生产类胡萝卜素c13中的应用综述

c13 -类伪胡萝卜素是类胡萝卜素裂解双加氧酶(CCDs)对类胡萝卜素的C9-C10 (C9'-C10')双键裂解的产物。类胡萝卜素裂解双加氧酶1 (CCD1)亚家族在微生物细胞工厂中持续生产c13 -类胡萝卜素，作为食品和化妆品工业中的高价值香料和香料。然而，基于微生物的c13 -类伪胡萝卜素的商业化仍然受到CCD1性能不佳的限制，这严重限制了其从前体类胡萝卜素转化的效率。本文综述了类胡萝卜素ccd的分类及其裂解生成类胡萝卜素的方式。然后，我们着重讨论了微生物细胞工厂中c13 -类胡萝卜素生物合成的各种策略的进展，包括优化CCD1表达，提高CCD1的催化活性和底物特异性，加强底物通道，以及开发产油微生物宿主，这些策略已被证实可以提高类胡萝卜素的转化率。最后，讨论了目前面临的挑战和未来的发展方向，以提高ccd在类胡萝卜素c13生物制造中的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.