Microglia modulate sleep/wakefulness under baseline conditions and under acute social defeat stress in adult mice

IF 2.4 4区 医学 Q3 NEUROSCIENCES
Kazuya Miyanishi , Noriko Hotta-Hirashima , Chika Miyoshi , Satsuki Hayakawa , Miyo Kakizaki , Satomi Kanno , Aya Ikkyu , Hiromasa Funato , Masashi Yanagisawa
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Abstract

Although sleep is tightly regulated by multiple neuronal circuits in the brain, nonneuronal cells such as glial cells have been increasingly recognized as crucial sleep regulators. Recent studies have shown that microglia may act to maintain wakefulness. Here, we investigated the possible involvement of microglia in the regulation of sleep quantity and quality under baseline and stress conditions through electroencephalography (EEG)/electromyography (EMG) recordings, and by employing pharmacological methods to eliminate microglial cells in the adult mouse brain. We found that severe microglial depletion induced by the colony-stimulating factor 1 receptor (CSF1R) antagonist PLX5622 (PLX) reversibly decreased the total wake time and the wake episode duration and increased the EEG slow-wave power during wakefulness under baseline conditions. To examine the role of microglia in sleep/wake regulation under mental stress, we used the acute social defeat stress (ASDS) paradigm, an ethological model for psychosocial stress. Sleep analysis under ASDS revealed that microglial depletion exacerbated the stress-induced decrease in the total wake time and increase in anxiety-like behaviors in the open field test. These results demonstrate that microglia actively modulate sleep quantity and architecture under both baseline and stress conditions. Our findings suggest that microglia may potentially provide resilience against acute psychosocial stress by regulating restorative sleep.

小胶质细胞在基线条件下和急性社会失败应激下调节成年小鼠的睡眠/觉醒。
虽然睡眠是由大脑中的多个神经元回路严格调节的,但神经胶质细胞等非神经元细胞越来越被认为是至关重要的睡眠调节细胞。最近的研究表明,小胶质细胞可能起到维持清醒的作用。在这里,我们通过脑电图(EEG)/肌电图(EMG)记录,并采用药理学方法消除成年小鼠大脑中的小胶质细胞,研究了小胶质细胞在基线和应激条件下调节睡眠数量和质量的可能参与。我们发现,在基线条件下,由集落刺激因子1受体(CSF1R)拮抗剂PLX5622 (PLX)诱导的严重小胶质细胞耗竭可可逆地减少总清醒时间和清醒发作持续时间,并增加清醒时脑电图慢波功率。为了研究小胶质细胞在精神压力下睡眠/觉醒调节中的作用,我们使用了急性社会失败压力(ASDS)范式,这是一种心理社会压力的行为学模型。ASDS下的睡眠分析显示,小胶质细胞耗损加剧了应激导致的总清醒时间的减少和开放性测试中焦虑样行为的增加。这些结果表明,在基线和应激条件下,小胶质细胞积极调节睡眠量和结构。我们的研究结果表明,小胶质细胞可能通过调节恢复性睡眠来提供对急性社会心理压力的恢复能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Neuroscience Research
Neuroscience Research 医学-神经科学
CiteScore
5.60
自引率
3.40%
发文量
136
审稿时长
28 days
期刊介绍: The international journal publishing original full-length research articles, short communications, technical notes, and reviews on all aspects of neuroscience Neuroscience Research is an international journal for high quality articles in all branches of neuroscience, from the molecular to the behavioral levels. The journal is published in collaboration with the Japan Neuroscience Society and is open to all contributors in the world.
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