Increased levels of IL-10 in the spleen after induction of pili protein 65.5 kDa Klebsiella pneumoniae

IF 0.5 Q4 EDUCATION, SCIENTIFIC DISCIPLINES

Pharmacy Education Pub Date : 2023-10-10 DOI:10.46542/pe.2023.234.304310

Dini Agustina, None Diana Chusna Mufida, None Enny Suswati, None M. Ali Shodikin, None Bagus Hermansyah, None Ajeng Samrotu Sa'adah, None Tio Wisnu Pradana Putra, None Samudra Ayu

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Abstract

Background: One of the gram-negative bacteria often found as the cause of nosocomial infections is Klebsiella pneumoniae. This bacterium has a mortality rate of 28.3% due to ESBL strains that cause resistance to several antibiotics and the absence of a vaccine as a preventive measure. Objective: To determine the level of IL-10 in the spleen after induction of the protein pili K. pneumoniae 65.5 kDa in mice. Method: This study used mice spleen samples from 21 mice aged six to eight weeks in an experimental investigation with a randomised posttest-only control group design. There are three groups, K1 as a PBS-given control, 65.5 kDa antigen protein pills + Freund’s adjuvants are given to K2, and Freund's adjuvants are given to K3. Interleukin-10 concentrations were measured using the ELISA and analysed by a one-way ANOVA assay. Result: The research showed significant differences in each group at IL-10 levels after administration of the pili protein K. pneumoniae using one-way ANOVA (p = 0.036). The treatment group had the highest average levels of IL-10, so there was an increased association between exposure to pili protein and IL-10 levels in rats. A significant increase in IL-10 levels was found in the treatment group compared to the control and adjuvant groups. Conclusion: Induction of protein pili Klebsiella pneumoniae 65.5 kDa increases IL-10 levels in spleen mice.

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肺炎克雷伯菌菌毛蛋白65.5 kDa诱导后脾脏IL-10水平升高

背景:常见的引起医院感染的革兰氏阴性菌之一是肺炎克雷伯菌。这种细菌的死亡率为28.3%，因为ESBL菌株对几种抗生素产生耐药性，而且缺乏疫苗作为预防措施。目的:探讨肺炎克雷伯菌蛋白65.5 kDa诱导小鼠脾组织中IL-10水平的变化。方法:以21只6 ~ 8周龄小鼠脾脏为研究对象，采用随机后测对照组设计。有三组，K1作为pbs给予对照，K2给予65.5 kDa抗原蛋白丸+ Freund’s佐剂，K3给予Freund’s佐剂。白细胞介素-10浓度采用ELISA法测定，单因素方差分析。结果:经单因素方差分析，各组注射肺炎克雷伯菌菌毛蛋白后IL-10水平差异有统计学意义(p = 0.036)。治疗组的IL-10平均水平最高，因此暴露于毛蛋白和大鼠IL-10水平之间的相关性增加。与对照组和辅助组相比，治疗组IL-10水平显著增加。结论:65.5 kDa肺炎克雷伯菌蛋白毛菌诱导脾脏小鼠IL-10水平升高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmacy Education EDUCATION, SCIENTIFIC DISCIPLINES-

CiteScore

0.80

自引率

20.00%

发文量

174

期刊介绍： Pharmacy Education journal provides a research, development and evaluation forum for communication between academic teachers, researchers and practitioners in professional and pharmacy education, with an emphasis on new and established teaching and learning methods, new curriculum and syllabus directions, educational outcomes, guidance on structuring courses and assessing achievement, and workforce development. It is a peer-reviewed online open access platform for the dissemination of new ideas in professional pharmacy education and workforce development. Pharmacy Education supports Open Access (OA): free, unrestricted online access to research outputs. Readers are able to access the Journal and individual published articles for free - there are no subscription fees or ''pay per view'' charges. Authors wishing to publish their work in Pharmacy Education do so without incurring any financial costs.