A study of the stability of antioxidant activity of green tea extract powder with vitamin C

IF 0.5 Q4 EDUCATION, SCIENTIFIC DISCIPLINES

Pharmacy Education Pub Date : 2023-10-10 DOI:10.46542/pe.2023.234.159163

Djoko Agus Purwanto, Betria Dwi Agustin, Juni Ekowati

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Abstract

Background: Green tea contains Epigallocatechin gallate (EGCG), the highest antioxidant activity. EGCG is an unstable compound due to degradation. Vitamin C is thought to be able to regenerate EGCG radicals into normal forms and increase green tea's antioxidant activity. Objective: The aim of this research was to determine the effect of adding vitamin C in maintaining the stability of the antioxidant activity of green tea. Method: This research was carried out by using UV-Vis spectrophotometry to determine the rate of decreasing antioxidant activity at 0 hours, six hours, and 24 hours by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Result: IC50 values of green tea before and after the addition of vitamin C were obtained at 147.53 ppm and 97.75 ppm, respectively. The degradation rate of green tea before and after the addition of vitamin C was 0.4228 %/hour and 0.3231 %/hour, respectively. Conclusion: The antioxidant activity of green tea with vitamin C is greater than without vitamin C addition. The degradation rate of green tea with vitamin C is smaller than green tea only, so the authors suggest the addition of vitamin C to green tea extract powder products to increase the stability of its antioxidant activity.

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含维生素C绿茶提取物粉末抗氧化活性稳定性的研究

背景:绿茶含有最高抗氧化活性的没食子儿茶素没食子酸酯(EGCG)。由于降解，EGCG是一种不稳定的化合物。维生素C被认为能够使EGCG自由基再生为正常形式，并增加绿茶的抗氧化活性。目的:研究添加维生素C对维持绿茶抗氧化活性稳定性的影响。方法:采用紫外-可见分光光度法，采用2,2-二苯基-1-苦味酰肼(DPPH)法测定其抗氧化活性在0、6、24 h时的下降速率。结果:添加维生素C前后绿茶的IC50值分别为147.53 ppm和97.75 ppm。添加维生素C前后绿茶的降解率分别为0.4228% /h和0.3231% /h。结论:添加维生素C的绿茶抗氧化活性高于未添加维生素C的绿茶。含有维生素C的绿茶的降解率比单纯的绿茶要小，因此作者建议在绿茶提取物粉产品中添加维生素C，以增加其抗氧化活性的稳定性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmacy Education EDUCATION, SCIENTIFIC DISCIPLINES-

CiteScore

0.80

自引率

20.00%

发文量

174

期刊介绍： Pharmacy Education journal provides a research, development and evaluation forum for communication between academic teachers, researchers and practitioners in professional and pharmacy education, with an emphasis on new and established teaching and learning methods, new curriculum and syllabus directions, educational outcomes, guidance on structuring courses and assessing achievement, and workforce development. It is a peer-reviewed online open access platform for the dissemination of new ideas in professional pharmacy education and workforce development. Pharmacy Education supports Open Access (OA): free, unrestricted online access to research outputs. Readers are able to access the Journal and individual published articles for free - there are no subscription fees or ''pay per view'' charges. Authors wishing to publish their work in Pharmacy Education do so without incurring any financial costs.