The optimisation and standardisation of Indonesian house dust mites allergenic extract as a desensitising agent

IF 0.5 Q4 EDUCATION, SCIENTIFIC DISCIPLINES

Pharmacy Education Pub Date : 2023-10-10 DOI:10.46542/pe.2023.234.137139

Yusuf Alif Pratama, Honey Dzikri Marhaeny, Salsabilla Madudari Kasatu, Lutfiatur Rohmah, Ahmad Dzulfikri Nurhan, Mahardian Rahmadi, Junaidi Khotib

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引用次数: 0

Abstract

Background: The different sequences of the allergen protease can induce different immunogenicity for the development of immunotherapy. Dermatophagoides pteronyssinus that has Der p1 as major protein will be developed as local allergen to be immunotherapy for treating rhinitis allergy. Objective: This study aims to optimize and standardize the local Indonesian House Dust Mites Allergenic Extract from the harvesting into the extraction process. Method: The harvesting media used is corn flour placed in certain conditions to produce the mites. After harvesting the flour in different weeks, the flour was extracted and the lipid content and Der p1 assay were measured. Result: The result of the study is that the 3 weeks-harvesting time came higher for those parameters than the other harvesting time (p < 0.05). Conclusion: Thus, it was concluded that the standardized harvesting and extraction process had been developed to produce the standardised Indonesian House Dust Mites Allergenic Extract.

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印尼屋尘螨致敏提取物脱敏剂的优化与标准化

背景:不同的变应原蛋白酶序列可诱导不同的免疫原性，促进免疫治疗的发展。以Der p1为主要蛋白的翼状棘球蚴将作为局部变应原发展成为治疗鼻炎过敏的免疫疗法。目的:优化和规范印尼当地屋尘螨致敏提取物从采收到提取的工艺流程。方法:采用玉米粉作为采收介质，在一定条件下生产螨虫。在不同周收获面粉后，提取面粉，测定其脂质含量和Der p1含量。结果:3周采收期对这些参数的影响均高于其他采收期(p <0.05)。结论:建立了标准化的采收提取工艺，可生产标准化的印尼屋尘螨致敏提取物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmacy Education EDUCATION, SCIENTIFIC DISCIPLINES-

CiteScore

0.80

自引率

20.00%

发文量

174

期刊介绍： Pharmacy Education journal provides a research, development and evaluation forum for communication between academic teachers, researchers and practitioners in professional and pharmacy education, with an emphasis on new and established teaching and learning methods, new curriculum and syllabus directions, educational outcomes, guidance on structuring courses and assessing achievement, and workforce development. It is a peer-reviewed online open access platform for the dissemination of new ideas in professional pharmacy education and workforce development. Pharmacy Education supports Open Access (OA): free, unrestricted online access to research outputs. Readers are able to access the Journal and individual published articles for free - there are no subscription fees or ''pay per view'' charges. Authors wishing to publish their work in Pharmacy Education do so without incurring any financial costs.