Taylan Topal, Asli Card, Andrew D. Mackenzie, Kirill Lagutin, Susan N. Marshall, Adam H. Cumming, Daniel P. Killeen
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引用次数: 0
Abstract
‘Type V' natural deep eutectic solvents (TV NADES) are a novel class of solvent media with many potential ‘green chemistry’ applications. Here, we assess the potential of two TV NADES: menthol:carvacrol (M:C) and menthol:thymol (M:T), and a single terpenoid extraction media carvacrol (C), as media from total lipid extraction of three compositionally diverse, freeze-dried marine tissues: hoki (Macruronus novaezelandaiae), jack mackerel (Trachurus declivis) and green-lipped mussel (kuku, Perna canaliculus). Room temperature extraction yields into these media were benchmarked relative to lipid yields from chloroform-methanol-water extractions. Relative extraction yields using the M:C and M:T media were (both) 87% for jack mackerel, 85%–87% for hoki, and 43%–47% for green-lipped mussels. Comprehensive chemical analyses of TV NADES lipid extracts suggested that neutral lipids were extracted in greater yield than phospholipids, and that phosphatidylinositol, lysophospholipids and (in the case of mussels) ceramideaminoethylphosphonates were particularly resistant to extraction into C, M:C and M:T. C—a thymol isomer that, unlike menthol and thymol is a liquid at room temperature—appeared to perform (marginally) better than M:C and M:T systems, implying there may be no advantage to using the TV NADES over a single terpenoid with similar chemistry for total lipid extraction.
期刊介绍：
The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate.
JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of yeartoyear, environmental, and/ or cultivar variations through use of appropriate statistical analyses.
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