Biochar preparation and its effects with reduced compound fertilizer on nutrients, phenolic acid and fungal community in tobacco rhizosphere soil

Abstract

Biochar is a carbon-rich soil conditioner produced from pyrolysis of biomass, it has been widely used to enhance soil quality because of its physical adsorption as well as water and fertilizer conservation functions. This work aimed to improve the soil quality of continuously cropped flue-cured tobacco fields using biochar prepared from agricultural waste. To explore the impact of reduced compound fertilizer with biochar application on nutrients, phenolic acid contents and fungi diversity in the rhizosphere soil, 4 treatments were set: regular compound fertilizer application (T1), and biochar with reduced compound fertilizer in different proportions (T2, T3, T4, with 100, 75, 50% of compound fertilizer, respectively). The physicochemical properties of the prepared biochar were characterized and observed using electron microscopy. The results indicated that, a noticeable increase in the content of soil organic matter (SOM) and soil organic carbon (SOC) in T2 treatment compared to T1 treatment. Moreover, T2 treatment demonstrated a significant improvement in the contents of alkali-hydrolyzable nitrogen (AN), available phosphorus (AP), and available potassium (AK), with increases of 9.29%, 15.85% and 25.42% compared to T1 treatment, respectively. While a gradual decrease in soil AN, AP, and AK content was observed with the reduction of compound fertilizer application (T2–T4), there was no obvious difference between T3 and T1. For total phenolic acid, the content in T2 treatment significantly decreased by 35.99% compared to T1 treatment, while T3 and T4 treatments showed significant reductions of 15.30 and 18.73% respectively, compared to T2 treatment. Biochar application could enhance the fungal community’s abundance and diversity in the rhizosphere soil. Fungal community exhibited the highest richness under T3 treatment, while the relative abundance of Fusarium and Mortierella reduced as the decrease of compound fertilizer (T2–T4). In conclusion, the reduced compound fertilizer with biochar application could reduce nutrient loss, phenolic acids accumulation, and improving the abundance of fungal community in the rhizosphere soil. This paper provides a reference for biochar combined with compound fertilizer to improve soil from the regulation of allelochemicals and soil fungi.