Jinxz Pollard-Flamand, Julie Boulé, Miranda Hart, José Ramón Úrbez-Torres
{"title":"Biological Control of Botryosphaeria Dieback of Grapevines in British Columbia, Canada","authors":"Jinxz Pollard-Flamand, Julie Boulé, Miranda Hart, José Ramón Úrbez-Torres","doi":"10.5344/ajev.2023.23052","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> <h3>Background and goals</h3> Botryosphaeria dieback is a grapevine trunk disease responsible for significant economic losses to grape producers worldwide. There are currently no products registered in Canada to control grapevine trunk diseases, and sustainable production has become a focus of the Canadian grape and wine industry. Therefore, the objectives of this research were to evaluate locally sourced <i>Trichoderma</i> spp. as biocontrol of Botryosphaeria dieback fungi in the field, to compare <i>Trichoderma</i> spp. against commercial products registered in other countries, and to determine optimum temperatures for conidial germination of <i>Trichoderma</i> spp. found in British Columbia (BC). <h3>Methods and key findings</h3> Detached cane assays under greenhouse conditions and field trials in a Merlot vineyard during two growing seasons assessed <i>Trichoderma</i> spp. from BC and commercial products as pruning wound protectants against <i>Diplodia seriata</i> and <i>Neofusicoccum parvum</i> over time. The <i>Trichoderma</i>-based treatments developed in this study provided pruning wound protection for up to 60 days after treatment and performed better or similarly to commercial products. Germination studies showed conidia of <i>Trichoderma canadense</i> germinated faster at lower temperatures than the other <i>Trichoderma</i> spp. <h3>Conclusions and significance</h3> This represents the first study evaluating locally sourced <i>Trichoderma</i> spp. as protection against grapevine trunk disease fungi under field conditions in Canada, and shows the potential that these endophytic isolates have as pruning wound protectants over time. This study also screened commercial products to manage grapevine trunk diseases for the first time in Canada, providing key data to support product registration. The discovery of faster conidial germination of <i>T. canadense</i> at lower temperatures deserves further investigation, as this species could be better adapted and thus provide more effective pruning wound protection when applied at the colder temperatures experienced in BC during the pruning season.","PeriodicalId":7461,"journal":{"name":"American Journal of Enology and Viticulture","volume":"46 1","pages":"0"},"PeriodicalIF":2.2000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"American Journal of Enology and Viticulture","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5344/ajev.2023.23052","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background and goals
Botryosphaeria dieback is a grapevine trunk disease responsible for significant economic losses to grape producers worldwide. There are currently no products registered in Canada to control grapevine trunk diseases, and sustainable production has become a focus of the Canadian grape and wine industry. Therefore, the objectives of this research were to evaluate locally sourced Trichoderma spp. as biocontrol of Botryosphaeria dieback fungi in the field, to compare Trichoderma spp. against commercial products registered in other countries, and to determine optimum temperatures for conidial germination of Trichoderma spp. found in British Columbia (BC).
Methods and key findings
Detached cane assays under greenhouse conditions and field trials in a Merlot vineyard during two growing seasons assessed Trichoderma spp. from BC and commercial products as pruning wound protectants against Diplodia seriata and Neofusicoccum parvum over time. The Trichoderma-based treatments developed in this study provided pruning wound protection for up to 60 days after treatment and performed better or similarly to commercial products. Germination studies showed conidia of Trichoderma canadense germinated faster at lower temperatures than the other Trichoderma spp.
Conclusions and significance
This represents the first study evaluating locally sourced Trichoderma spp. as protection against grapevine trunk disease fungi under field conditions in Canada, and shows the potential that these endophytic isolates have as pruning wound protectants over time. This study also screened commercial products to manage grapevine trunk diseases for the first time in Canada, providing key data to support product registration. The discovery of faster conidial germination of T. canadense at lower temperatures deserves further investigation, as this species could be better adapted and thus provide more effective pruning wound protection when applied at the colder temperatures experienced in BC during the pruning season.
期刊介绍:
The American Journal of Enology and Viticulture (AJEV), published quarterly, is an official journal of the American Society for Enology and Viticulture (ASEV) and is the premier journal in the English language dedicated to scientific research on winemaking and grapegrowing. AJEV publishes full-length research papers, literature reviews, research notes, and technical briefs on various aspects of enology and viticulture, including wine chemistry, sensory science, process engineering, wine quality assessments, microbiology, methods development, plant pathogenesis, diseases and pests of grape, rootstock and clonal evaluation, effect of field practices, and grape genetics and breeding. All papers are peer reviewed, and authorship of papers is not limited to members of ASEV. The science editor, along with the viticulture, enology, and associate editors, are drawn from academic and research institutions worldwide and guide the content of the Journal.