Amelioration of dextran sodium sulphate-induced colitis in mice by treatment with Lactobacillus rhamnosus and Lactobacillus reuteri: intraspecific and interspecific patterns

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness Pub Date : 2024-09-01 DOI:10.26599/FSHW.2022.9250227

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Abstract

Lactobacillus rhamnosus (Rh) and Lactobacillus reuteri (Re) are well-known probiotic species in inflammatory bowel disease (IBD) research. The variations between these species’ efficacy against colitis, and their model of action in this regard, are intriguing and enable treatment to be individually tailored to patients. In this study, four strains each of Rh and Re were isolated from fecal samples and their draft genomes were sequenced. The anti-colitis activities of both strains involved various aspects of intestinal immune, physical, chemical, and biological barrier function. Strikingly, the tested strains exhibited considerable interspecies and intraspecies specificity in colitis amelioration. Rh strains significantly outperformed Re strains in terms of short-chain fatty acid synthesis. Nevertheless, Re strains were more effective than Rh strains in inhibiting production of inflammatory factors; promoting production of intestinal mucus, antimicrobial peptides, and tight junction proteins; and supporting the stem cell compartment. This accounts for the anti-colitis outcomes of Re strains being superior to those of Rh strains. In addition, the effective Rh and Re strains were found to express high concentrations of specific carbohydrate metabolism- and prophage-related genes, respectively. Taken together, the results of this study could assist researchers in developing effective therapies for IBD.

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鼠李糖乳杆菌和沙门氏菌治疗小鼠右旋糖酐硫酸钠诱发的结肠炎：种内和种间模式

鼠李糖乳杆菌（Rh）和罗伊氏乳杆菌（Re）是炎症性肠病（IBD）研究中著名的益生菌。这两种益生菌对结肠炎的疗效及其作用模式之间的差异十分有趣，可为患者提供个性化治疗。在这项研究中，从粪便样本中分离出了 Rh 和 Re 各四株菌株，并对它们的基因组草案进行了测序。这两种菌株的抗结肠炎活性涉及肠道免疫、物理、化学和生物屏障功能的各个方面。引人注目的是，受试菌株在改善结肠炎方面表现出相当大的种间和种内特异性。在短链脂肪酸合成方面，Rh 菌株明显优于 Re 菌株。然而，在抑制炎症因子的产生、促进肠粘液、抗菌肽和紧密连接蛋白的产生以及支持干细胞区系方面，Re 菌株比 Rh 菌株更有效。这就是 Re 菌株抗结肠炎效果优于 Rh 菌株的原因。此外，还发现有效的 Rh 和 Re 菌株分别表达高浓度的特定碳水化合物代谢基因和噬菌体相关基因。综上所述，这项研究的结果有助于研究人员开发治疗肠道疾病的有效疗法。

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来源期刊

Food Science and Human Wellness Agricultural and Biological Sciences-Food Science

CiteScore

8.30

自引率

5.70%

发文量

审稿时长

28 days

期刊介绍： Food Science and Human Wellness is an international peer-reviewed journal that provides a forum for the dissemination of the latest scientific results in food science, nutriology, immunology and cross-field research. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. By their effort, it has been developed to promote the public awareness on diet, advocate healthy diet, reduce the harm caused by unreasonable dietary habit, and directs healthy food development for food industrial producers.