Polymerized retinoic acid nanoparticles modulate neurorestorative effects induced by bone marrow stromal cells in rats after anesthesia

Abstract

This study regulated the induced differentiation of bone marrow stromal cells (BMSCs) in rats after anesthesia and explored its effect on nerve repair. The effect of MSC-induced nerve repair was analyzed. The scores of cell-intervention group (5.43± 1.35), nano-intervention group (4.43± 1.36) and nano-+cell-intervention group (4.45±1.49) were significantly lower on 28th day than control group (9.99±1.40), among which, the nano+cell intervention group had lowest score ( P <0.05).The cell intervention (11.35±1.23), nanometer intervention (14.81±1.55) and nano+cell intervention groups (15.96±1.45) had significantly lower score than control group (6.42± 1.46), with lowest score in the nano+ cell intervention group ( P < 0.05). The expressions of glial fibrillary acidic protein (GFAP) and NeuN proteins in the treatment group were significantly decreased, with lowest expression in the nano+cell intervention group ( P <0.05). Average optical density of bFGF and EGF after treatment was significantly elevated, with highest density values in the nano+cell intervention group ( P <0.05). Using retinoic acid polymeric nanoparticles to regulate MSCs differentiation can make retinoic acid bind to neuronal receptors, promoting axon growth, and improving nerve function and motor function. It can reduce downregulate GFAP and NeuN, increase the bFGF and EGF level, which can be used as a new target marker. With the deepening research on nanoparticles, retinoic acid nanoparticles will have broad application prospects.