Lacticaseibacillus rhamnosus Fmb14 ameliorates hyperuricemia-induced hepatocyte pyroptosis via NLRP3 inflammasome cascade inhibition

IF 5.6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Hongyuan Zhao , Xiaoyu Chen , Li Zhang , Fanqiang Meng , Libang Zhou , Zhaoxin Lu , Yingjian Lu
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Abstract

Hyperuricemia is a high-risk factor for the development of gout and renal fibrosis, but the adverse effects of hyperuricemia on the liver have been seriously neglected. This research investigated the ameliorating effect of Lacticaseibacillus rhamnosus Fmb14 on hyperuricemia induced liver dysfunction both in vitro and in vivo. Cell free extracts of high dose L. rhamnosus Fmb14 treatment reduced the death rate of HepG2 cell lines from 24.1 % to 14.9 % by inhibiting NLRP3 recruitment, which was mainly activated by reactive oxygen species release and mitochondrial membrane potential disorder. In purine dietary induced hyperuricemia (PDIH) mice model, liver oedema and pyroptosis were ameliorated after L. rhamnosus Fmb14 administration through downregulating the expression levels of NLRP3, caspase-1 and gasdermin-D from 1.61 to 0.86, 3.15 to 1.01 and 5.63 to 2.02, respectively. L. rhamnosus Fmb14 administration restored mitochondrial inner membrane protein (MPV17) and connexin 43 from 2.83 and 0.73 to 0.80 and 0.98 respectively in PDIH mice, indicating that dysbiosis of mitochondrial membrane potential was restored in liver. Intriguingly, PDIH pyroptosis stimulates the process of apoptosis, which leads to severe leakage of hepatocytes, and both of pyroptosis and apoptosis were decreased after L. rhamnosus Fmb14 treatment. Therefore, L. rhamnosus Fmb14 is a promising biological resource to maintain homeostasis of the liver in hyperuricemia and the prevention of subsequent complications.

Abstract Image

鼠李糖乳杆菌 Fmb14 通过抑制 NLRP3 炎症组级联改善高尿酸血症诱导的肝细胞脓毒症
高尿酸血症是痛风和肾脏纤维化的高危因素,但高尿酸血症对肝脏的不良影响却被严重忽视。本研究探讨了鼠李糖乳杆菌 Fmb14 在体外和体内对高尿酸血症引起的肝功能异常的改善作用。高剂量鼠李糖乳杆菌 Fmb14 的细胞游离提取物通过抑制 NLRP3 招募,将 HepG2 细胞系的死亡率从 24.1% 降至 14.9%,而 NLRP3 招募主要由活性氧释放和线粒体膜电位紊乱激活。在嘌呤饮食诱导的高尿酸血症(PDIH)小鼠模型中,鼠李糖 Fmb14 通过下调 NLRP3、caspase-1 和 gasdermin-D 的表达水平,分别从 1.61 降至 0.86、3.15 降至 1.01 和 5.63 降至 2.02,从而改善了肝水肿和脓毒症。鼠李糖 Fmb14 使 PDIH 小鼠的线粒体内膜蛋白(MPV17)和连接蛋白 43 分别从 2.83 和 0.73 降至 0.80 和 0.98,这表明肝脏中线粒体膜电位失调得到了恢复。耐人寻味的是,PDIH 热解会刺激肝细胞凋亡,导致严重的肝细胞渗漏,而鼠李糖 Fmb14 处理后,热解和凋亡均减少。因此,鼠李糖 Fmb14 是维持高尿酸血症肝脏平衡和预防后续并发症的一种很有前景的生物资源。
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来源期刊
Food Science and Human Wellness
Food Science and Human Wellness Agricultural and Biological Sciences-Food Science
CiteScore
8.30
自引率
5.70%
发文量
80
审稿时长
28 days
期刊介绍: Food Science and Human Wellness is an international peer-reviewed journal that provides a forum for the dissemination of the latest scientific results in food science, nutriology, immunology and cross-field research. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. By their effort, it has been developed to promote the public awareness on diet, advocate healthy diet, reduce the harm caused by unreasonable dietary habit, and directs healthy food development for food industrial producers.
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