Repeatedly usable bio-molecule cantilever sensors with a coordination-bond breaking and re-linking technique

Abstract

This paper reports a method for recycled immobilizing and erasing antibody at micro-cantilever surface by a coordination-bond breaking and re-linking technique. With the new method, the sensor can be re-used to immuno-detect bio-molecules for many times. By our sensing experiment to killed vaccine of Bacillus Anthracis (BA) spores with concentration of 2000 spores/ml for three times, the target loaded on the cantilever surface causes a decrease of its frequency shift by 106 Hz, 100 Hz and 107 Hz, respectively. The biosensor detection sensitivity shows no decrease after multiple sensing cycles.