Pluchea indica Leaf Extract Alleviates Dyslipidemia and Hepatic Steatosis by Modifying the Expression of Lipid Metabolism-Related Genes in Rats Fed a High Fat-High Fructose Diet.

IF 1.6 Q3 FOOD SCIENCE & TECHNOLOGY
Patcharin Singdam, Jarinyaporn Naowaboot, Laddawan Senggunprai, Kampeebhorn Boonloh, Patchareewan Pannangpetch
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Abstract

This study evaluated the effect of Pluchea indica leaf extract (PIE) on dyslipidemia and lipid accumulation in the liver, emphasizing its molecular mechanisms in regulating lipid metabolism in rats fed a high fat-high fructose diet (HFFD). Male rats were fed HFFD (40% lard and 20% fructose) for ten weeks. They were then divided into four groups receiving distilled water, PIE (100 or 300 mg/kg/d), and pioglitazone (10 mg/kg/d) for a further six weeks, during which the HFFD was continued. After the experiment, fasting blood glucose (FBG), oral glucose tolerance (OGT), serum insulin and leptin levels, lipid profiles, and hepatic triglyceride content were measured. Histological examination and expression levels of lipid metabolism-related genes in the liver were measured. HFFD-fed rats indicated a significantly increased FBG, serum leptin, and homeostasis model assessment of insulin resistance (HOMA-IR) scores with impaired OGT and dyslipidemia compared to rats fed a normal diet. PIE significantly reduced FBG, serum leptin, and HOMA-IR scores and improved OGT. Additionally, PIE significantly improved dyslipidemia and decreased serum-free fatty acids and liver triglyceride content. Hepatic histological examination showed a marked reduction lipid accumulation in relation to HFFD controls. Interestingly, PIE significantly downregulated the expression of lipid synthesis-related genes and upregulated the expression of fatty-acid oxidation-related genes. In conclusion, PIE alleviates dyslipidemia and hepatic steatosis in HFFD rats plausibly by increasing insulin resistance and modifying the gene expression associated with lipid metabolism. PIE may be used as preventive nutrition for dyslipidemia and hepatic steatosis.

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梅子叶提取物通过改变高脂高果糖饮食大鼠脂质代谢相关基因的表达来缓解血脂异常和肝脏脂肪变性。
本研究评价了紫果叶提取物(PIE)对高脂高果糖饮食大鼠肝脏血脂异常和脂质积累的影响,强调其调节脂质代谢的分子机制。雄性大鼠喂食HFFD(40%猪油和20%果糖)10周。然后将他们分为四组,分别给予蒸馏水、PIE(100或300 mg/kg/d)和吡格列酮(10 mg/kg/d),持续六周,期间继续进行HFFD。实验结束后,测定空腹血糖(FBG)、口服葡萄糖耐量(OGT)、血清胰岛素和瘦素水平、血脂和肝脏甘油三酯含量。组织学检查及肝脏脂质代谢相关基因的表达水平。与正常饮食的大鼠相比,hffd喂养的大鼠FBG、血清瘦素和胰岛素抵抗(HOMA-IR)动态平衡模型评估评分显著增加,同时OGT受损和血脂异常。PIE显著降低了FBG、血清瘦素和HOMA-IR评分,并改善了OGT。此外,PIE显著改善血脂异常,降低血清游离脂肪酸和肝脏甘油三酯含量。肝脏组织学检查显示,与HFFD对照组相比,脂质积累明显减少。有趣的是,PIE显著下调脂质合成相关基因的表达,上调脂肪酸氧化相关基因的表达。综上所述,PIE可能通过增加胰岛素抵抗和改变脂质代谢相关基因表达来减轻HFFD大鼠的血脂异常和肝脏脂肪变性。PIE可作为血脂异常和肝脂肪变性的预防性营养。
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来源期刊
Preventive Nutrition and Food Science
Preventive Nutrition and Food Science Agricultural and Biological Sciences-Food Science
CiteScore
3.40
自引率
0.00%
发文量
35
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