In vivo direct imaging of neuronal activity at high temporospatial resolution

IF 44.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2022-10-13 DOI:10.1126/science.abh4340

Phan Tan Toi, Hyun Jae Jang, Kyeongseon Min, Sung-Phil Kim, Seung-Kyun Lee, Jongho Lee, Jeehyun Kwag, Jang-Yeon Park

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引用次数: 31

Abstract

There has been a long-standing demand for noninvasive neuroimaging methods that can detect neuronal activity at both high temporal and high spatial resolution. We present a two-dimensional fast line-scan approach that enables direct imaging of neuronal activity with millisecond precision while retaining the high spatial resolution of magnetic resonance imaging (MRI). This approach was demonstrated through in vivo mouse brain imaging at 9.4 tesla during electrical whisker-pad stimulation. In vivo spike recording and optogenetics confirmed the high correlation of the observed MRI signal with neural activity. It also captured the sequential and laminar-specific propagation of neuronal activity along the thalamocortical pathway. This high-resolution, direct imaging of neuronal activity will open up new avenues in brain science by providing a deeper understanding of the brain’s functional organization, including the temporospatial dynamics of neural networks.

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以高时间空间分辨率对神经元活动进行体内直接成像

长期以来，人们一直需要能以高时间分辨率和高空间分辨率检测神经元活动的无创神经成像方法。我们提出了一种二维快速线扫描方法，它能以毫秒级的精度对神经元活动进行直接成像，同时保留了磁共振成像（MRI）的高空间分辨率。这种方法通过在 9.4 特斯拉下对小鼠脑部进行电须垫刺激时的活体成像进行了验证。体内尖峰记录和光遗传学证实了观察到的磁共振成像信号与神经活动高度相关。它还捕捉到了神经元活动沿着丘脑皮质通路的顺序和层状特异性传播。这种神经元活动的高分辨率直接成像技术将为脑科学开辟新的途径，让人们更深入地了解大脑的功能组织，包括神经网络的时空动态。

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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