Broadband Dielectric Spectroscopy as a Potential Label-Free Method to Rapidly Verify Ultraviolet-C Radiation Disinfection.

IF 1.5 4区 工程技术
Yaw S Obeng, Brian J Nablo, Darwin R Reyes, Dianne L Poster, Michael T Postek
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引用次数: 0

Abstract

Microwave (MW) sensing offers noninvasive, real-time detection of the electromagnetic properties of biological materials via the highly concentrated electromagnetic fields, for which advantages include wide bandwidth, small size, and cost-effective fabrication. In this paper, we present the application of MW broadband dielectric spectroscopy (BDS) coupled to a fabricated biological thin film for evaluating ultraviolet-C (UV-C) exposure effects. The BDS thin film technique could be deployed as a biological indicator for assessing whole-room UV-C surface disinfection. The disinfection process is monitored by BDS as changes in the electrical properties of surface-confined biological thin films photodegraded with UV-C radiation. Fetal bovine serum (FBS, a surrogate for protein) and bacteriophage lambda double-stranded deoxyribonucleic acid (dsDNA) were continuously monitored with BDS during UV-C radiation exposure. The electrical resistance of FBS films yielded promising yet imprecise readings, whereas the resistance of dsDNA films discernibly decreased with UV-C exposure. The observations are consistent with the expected photo-oxidation and photodecomposition of protein and DNA. While further research is needed to characterize these measurements, this study presents the first application of BDS to evaluate the electrical properties of solid-state biological thin films. This technique shows promise toward the development of a test method and a standard biological test to determine the efficacy of UV-C disinfection. Such a test with biological indicators could easily be applied to hospital rooms between patient occupancy for a multipoint evaluation to determine if a room meets a disinfection threshold set for new patients.

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宽带介电光谱法是一种潜在的无标签方法,可用于快速验证紫外线-C 辐射消毒。
微波(MW)传感可通过高度集中的电磁场对生物材料的电磁特性进行非侵入式实时检测,其优点包括带宽宽、体积小和制造成本低。在本文中,我们介绍了将微波宽带介电光谱(BDS)与制备的生物薄膜相结合,用于评估紫外线-C(UV-C)照射效应的应用。BDS 薄膜技术可用作评估整个房间紫外线-C 表面消毒效果的生物指标。BDS 监测的消毒过程是在紫外线-C 辐射下光降解的表面封闭生物薄膜的电特性变化。在紫外线-C 辐射照射期间,用 BDS 连续监测胎牛血清(FBS,蛋白质的替代物)和噬菌体 lambda 双链脱氧核糖核酸(dsDNA)。FBS 薄膜的电阻读数很有希望,但并不精确,而 dsDNA 薄膜的电阻则随着紫外线-C 的照射明显下降。这些观察结果与蛋白质和 DNA 的预期光氧化和光分解相一致。虽然还需要进一步研究来确定这些测量结果的特征,但本研究首次应用 BDS 评估了固态生物薄膜的电学特性。这项技术显示了开发测试方法和标准生物测试以确定紫外线-C 消毒效果的前景。这种带有生物指标的测试可以很容易地应用于病人入住期间的病房,进行多点评估,以确定病房是否达到为新病人设定的消毒阈值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
自引率
33.30%
发文量
10
期刊介绍: The Journal of Research of the National Institute of Standards and Technology is the flagship publication of the National Institute of Standards and Technology. It has been published under various titles and forms since 1904, with its roots as Scientific Papers issued as the Bulletin of the Bureau of Standards. In 1928, the Scientific Papers were combined with Technologic Papers, which reported results of investigations of material and methods of testing. This new publication was titled the Bureau of Standards Journal of Research. The Journal of Research of NIST reports NIST research and development in metrology and related fields of physical science, engineering, applied mathematics, statistics, biotechnology, information technology.
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