丁酸盐通过激活ERK/MAPK通路†促进C2C12成肌细胞增殖

IF 3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Molecular omics Pub Date : 2023-05-19 DOI:10.1039/D2MO00256F
Li Guan, Ziyi Cao, Ziyue Pan, Chao Zhao, Mengjuan Xue, Fan Yang and Jie Chen
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引用次数: 2

摘要

近年来,骨骼肌减少症引起了相当大的兴趣,因为与衰老相关的疾病构成了全球重大的公共卫生负担。营养补充剂作为治疗肌肉减少症的潜在工具受到了广泛关注。然而,具体的营养成分仍在研究中。本研究首先采用超高效液相色谱-串联质谱法(UPLC-MS/MS)测定老年肌肉减少症患者和健康老年人粪便中的短链脂肪酸(SCFAs)和肠道菌群水平。采用细胞活力检测、流式细胞术和转录组分析等方法,实验评价SCFA对C2C12细胞体外增殖的影响及其机制。结果表明,肌少症患者表现出丁酸盐水平下降。丁酸盐可能通过促进G1/S细胞周期转变来刺激C2C12肌细胞增殖。转录组学分析指出,在丁酸盐处理的细胞中,丝裂原活化蛋白激酶(MAPK)信号通路上调。此外,ERK/MAPK抑制剂联合使用可抑制上述增殖表型。我们的研究采用转录组学和代谢组学相结合的方法来研究微生物来源的丁酸盐产量对肌肉增殖的潜在影响,这可能表明营养补充剂具有保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Butyrate promotes C2C12 myoblast proliferation by activating ERK/MAPK pathway†

Butyrate promotes C2C12 myoblast proliferation by activating ERK/MAPK pathway†

Sarcopenia has garnered considerable interest in recent years as ageing-associated diseases constitute a significant worldwide public health burden. Nutritional supplements have received much attention as potential tools for managing sarcopenia. However, the specific nutrients responsible are still under-investigated. In the current study, we first determined the levels of short chain fatty acids (SCFAs) and intestinal flora in the feces of elderly sarcopenia subjects and elderly healthy individuals by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Then cell viability detection, flow cytometry and transcriptome analysis were adopted to experimentally evaluate the effect and the underlying mechanism of SCFA on C2C12 cells proliferation in vitro. The results suggested that patients with sarcopenia exhibited decreased levels of butyrate. And butyrate may stimulate C2C12 myocyte proliferation via promoting G1/S cell cycle transition. Transcriptomic analyses pointed to upregulation of the Mitogen-activated protein kinase (MAPK) signaling pathway in butyrate-treated cells. In addition, the above proliferative phenotypes could be suppressed by the combination of ERK/MAPK inhibitor. A combined transcriptomic and metabolomic approach was applied in our study to investigate the potential effect of microbiota-derived butyrate yield on muscular proliferation which may indicate a protective effect of nutritional supplements.

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来源期刊
Molecular omics
Molecular omics Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
5.40
自引率
3.40%
发文量
91
期刊介绍: Molecular Omics publishes high-quality research from across the -omics sciences. Topics include, but are not limited to: -omics studies to gain mechanistic insight into biological processes – for example, determining the mode of action of a drug or the basis of a particular phenotype, such as drought tolerance -omics studies for clinical applications with validation, such as finding biomarkers for diagnostics or potential new drug targets -omics studies looking at the sub-cellular make-up of cells – for example, the subcellular localisation of certain proteins or post-translational modifications or new imaging techniques -studies presenting new methods and tools to support omics studies, including new spectroscopic/chromatographic techniques, chip-based/array technologies and new classification/data analysis techniques. New methods should be proven and demonstrate an advance in the field. Molecular Omics only accepts articles of high importance and interest that provide significant new insight into important chemical or biological problems. This could be fundamental research that significantly increases understanding or research that demonstrates clear functional benefits. Papers reporting new results that could be routinely predicted, do not show a significant improvement over known research, or are of interest only to the specialist in the area are not suitable for publication in Molecular Omics.
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