新型抗脑缺血纳米材料:龙脑当归多糖脂质体的制备与应用。

IF 3.6 4区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Liposome Research Pub Date : 2023-06-01 DOI:10.1080/08982104.2022.2106240

Huang Ding, Ke Wang, Lin-Qin He, Xiao-Yu Yang, Hai-Hong Huang, Wen-Long Liu, Chen Xiao, Ze-Xuan Du, Lian- Yu, Wei Zhang

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引用次数: 2

摘要

目的:研究新型抗脑缺血纳米脂质体(龙脑当归多糖脂质体，BAPL)的制备，并验证其疗效和作用机制。方法:采用均匀试验设计，探讨BAPL的拟合组合。BAPL制剂的包封效率评价BAPL生物活性的粒径及表面电位评价大鼠脑缺血模型研究BAPL的疗效及作用机制。结果:(1)卵磷脂、胆固醇、AP质量和冰片质量的适宜组合分别为60 mg、60 mg、45 mg和5 mg。包封效率最高为80.4%，粒径为179.1 nm，表面zeta电位为-17.2 mV。符合纳米材料标准。(2)动物实验结果表明:BAPL组大鼠TTC染色梗死体积明显减小，脑组织中NF-κBp65、TLR-4、IL-8、IL-6、IL-1β表达水平明显降低，脑缺血再灌注后ZO-1、ZO-2、IL-10表达水平明显升高。结论:BAPL是一种新型的抗脑缺血纳米材料。

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A novel nano material for anti-cerebral ischaemia: preparation and application of borneol angelica polysaccharide liposomes.

Objective: To investigate the preparation of novel nanoliposomes (Borneol Angelica Polysaccharide Liposomes, BAPL) for anti-cerebral ischaemia and verify its curative effects and mechanism.

Methods: By applying a uniform experiment design to investigate the fitting combination of BAPL. Encapsulation Efficiency Evaluation of BAPL Preparation; Particle Size and Surface Potential Evaluation of BAPL Biological activity; Cerebral ischaemia models of rats Evaluation of BAPL curative effects and mechanism.

Results: (1) The fitting combination of lecithin, Cholesterol, AP mass and the borneol mass was 60 mg, 60 mg, 45 mg and 5 mg. the highest encapsulation efficiency was 80.4%, the particle size was 179.1 nm, and the surface zeta potential was -17.2 mV. It conforms to the nano-material standards. (2) The results of animal experiments show that: In the BAPL group, the infarct volume of TTC staining was significantly decreased, and the expression levels of NF-κBp65, TLR-4, IL-8, IL-6, IL-1β in brain tissue were significantly decreased, while the expression levels of ZO-1, ZO-2, IL-10 were significantly increased after cerebral ischaemia-reperfusion.

Conclusion: BAPL is a novel nano and effective material for anti-cerebral ischaemia.

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来源期刊

Journal of Liposome Research 生物-生化与分子生物学

CiteScore

10.50

自引率

2.30%

发文量

审稿时长

3 months

期刊介绍： The Journal of Liposome Research aims to publish original, high-quality, peer-reviewed research on the topic of liposomes and related systems, lipid-based delivery systems, lipid biology, and both synthetic and physical lipid chemistry. Reviews and commentaries or editorials are generally solicited and are editorially reviewed. The Journal also publishes abstracts and conference proceedings including those from the International Liposome Society. The scope of the Journal includes: Formulation and characterisation of systems Formulation engineering of systems Synthetic and physical lipid chemistry Lipid Biology Biomembranes Vaccines Emerging technologies and systems related to liposomes and vesicle type systems Developmental methodologies and new analytical techniques pertaining to the general area Pharmacokinetics, pharmacodynamics and biodistribution of systems Clinical applications. The Journal also publishes Special Issues focusing on particular topics and themes within the general scope of the Journal.