静态范围内ToF-SIMS与静态范围外混合SIMS(动态模式)对小鼠脑组织的比较研究。

IF 1.6 4区 医学 Q4 BIOPHYSICS
Biointerphases Pub Date : 2023-05-01 DOI:10.1116/6.0002613
Hyun Kyong Shon, Jin Gyeong Son, Sun Young Lee, Jeong Hee Moon, Ga Seul Lee, Kyoung-Shim Kim, Tae Geol Lee
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引用次数: 0

摘要

在脑退行性疾病的研究中,神经元的主要成分脂质的变化尤为重要,因为它们被用作病理变化的指标。一种灵敏测量生物分子,特别是脂类的方法是使用脉冲氩簇离子的飞行时间二次离子质谱法(ToF-SIMS)。在本研究中,我们使用配备脉冲氩簇初级离子的ToF-SIMS测量了正常小鼠脑组织中存在的生物分子,包括各种脂类。在ToF-SIMS测量结果的基础上,采用添加轨道阱质谱分析仪的ToF-SIMS混合系统(OrbiSIMS)对真实组织样品中的生物分子进行区域直接鉴定。为此,在静态限度内测量单个小鼠大脑组织样本的ToF-SIMS的结果与在静态限度外测量的OrbiSIMS的结果在分子谱分析方面的差异进行了比较。通过分析,选择正离子和负离子两种类型进行鉴定,OrbiSIMS MS/MS结果表明,正离子为甘油磷酸胆碱,负离子为甘油磷酸肌醇和硫脂(一种鞘脂)。然后,为了确认候选分子的鉴定,从镜像组织样品中提取脂质,并使用轨道rap质谱分析仪进行LC-MS/MS。结果发现,通过OrbiSIMS直接鉴定组织样品中分布在特定区域的候选分子群与提取样品的LC-MS/MS鉴定结果一致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison study of mouse brain tissue by using ToF-SIMS within static limits and hybrid SIMS beyond static limits (dynamic mode).

In the study of degenerative brain diseases, changes in lipids, the main component of neurons, are particularly important because they are used as indicators of pathological changes. One method for the sensitive measurement of biomolecules, especially lipids, is time-of-flight secondary ion mass spectrometry (ToF-SIMS) using pulsed argon cluster ions. In this study, biomolecules including various lipids present in normal mouse brain tissue were measured using ToF-SIMS equipped with pulsed argon cluster primary ions. Based on the ToF-SIMS measurement results, hybrid SIMS (OrbiSIMS), which is a ToF-SIMS system with the addition of an orbitrap mass analyzer, was used to directly identify the biomolecules by the region in the real tissue samples. For this, the results of ToF-SIMS, which measured the tissue samples from a single mouse brain within static limits, were compared with those from OrbiSIMS measured beyond the static limits in terms of the differences in molecular profiling. From this analysis, two types of positive and negative ions were selected for identification, with the OrbiSIMS MS/MS results indicating that the positive ions were glycerophosphocholine and the negative ions were glycerophosphoinositol and sulfatide, a sphingolipid. Then, to confirm the identification of the molecular candidates, lipids were extracted from mirror image tissue samples, and LC-MS/MS also using an orbitrap mass analyzer was performed. As a result, the direct identification of molecular candidate groups distributed in particular regions of the tissue samples via OrbiSIMS was found to be consistent with the identification results by LC-MS/MS for extracted samples.

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来源期刊
Biointerphases
Biointerphases 生物-材料科学:生物材料
自引率
0.00%
发文量
35
期刊介绍: Biointerphases emphasizes quantitative characterization of biomaterials and biological interfaces. As an interdisciplinary journal, a strong foundation of chemistry, physics, biology, engineering, theory, and/or modelling is incorporated into originated articles, reviews, and opinionated essays. In addition to regular submissions, the journal regularly features In Focus sections, targeted on specific topics and edited by experts in the field. Biointerphases is an international journal with excellence in scientific peer-review. Biointerphases is indexed in PubMed and the Science Citation Index (Clarivate Analytics). Accepted papers appear online immediately after proof processing and are uploaded to key citation sources daily. The journal is based on a mixed subscription and open-access model: Typically, authors can publish without any page charges but if the authors wish to publish open access, they can do so for a modest fee. Topics include: bio-surface modification nano-bio interface protein-surface interactions cell-surface interactions in vivo and in vitro systems biofilms / biofouling biosensors / biodiagnostics bio on a chip coatings interface spectroscopy biotribology / biorheology molecular recognition ambient diagnostic methods interface modelling adhesion phenomena.
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