{"title":"印度南部和东北部金黄色葡萄球菌临床分离株的抗生素耐药性、生物膜形成能力和克隆谱分析。","authors":"Archana Loganathan, Ramesh Nachimuthu","doi":"10.2478/abm-2022-0023","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>Staphylococcus aureus</i> is a pathogen endemic in India and sometimes deadly for patients in intensive care units.</p><p><strong>Objectives: </strong>To determine the antibiotic-resistance pattern, biofilm forming ability, and clonal type of <i>S. aureus</i> from isolates collected in Tamil Nadu (south) and the Mizoram (northeast) regions of India.</p><p><strong>Methods: </strong>We collected <i>S. aureus</i> isolates from diagnostic laboratories in Tamil Nadu and Mizoram. An antibiotic susceptibility test was performed according to Clinical Laboratory and Standards Institute methods. Antibiotic-resistant determinants such as <i>mecA</i>, <i>mecC</i>, <i>blaZ</i>, <i>vanA</i>, <i>vanB</i>, and <i>vanC</i> were confirmed by polymerase chain reaction (PCR). All isolates were further studied for biofilm forming ability. Enterobacterial repetitive intergenic consensus (ERIC)-PCR was used for clonal analysis.</p><p><strong>Results: </strong>A study of 206 clinical isolates showed 52.9% prevalence of methicillin-resistant <i>S. aureus</i> in Tamil Nadu and 49.4% in Mizoram. Minimum inhibitory concentration tests showed a high prevalence of 67% oxacillin resistance in isolates from Tamil Nadu and 49% in isolates from Mizoram. PCR showed 53% <i>mecA</i> in Tamil Nadu and 49% <i>mecA</i> in Mizoram. Vancomycin-intermediate resistance <i>S. aureus</i> (VISA) prevalence was lower in isolates from Tamil Nadu (4%) and Mizoram (5%). All methicillin-resistant <i>S. aureus</i> (MRSA) isolates formed biofilms. Clonal analysis revealed a genetic relatedness between the isolates.</p><p><strong>Conclusions: </strong>The prevalence of MRSA is high in the regions studied, with most of the clinical isolates being multidrug resistant. Adopting appropriate community-based preventive measures and establishing antimicrobial stewardship is highly recommended to minimize the dissemination in antibiotic resistance.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 4","pages":"191-199"},"PeriodicalIF":0.4000,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321179/pdf/","citationCount":"1","resultStr":"{\"title\":\"Antibiotic resistance, biofilm forming ability, and clonal profiling of clinical isolates of <i>Staphylococcus aureus</i> from southern and northeastern India.\",\"authors\":\"Archana Loganathan, Ramesh Nachimuthu\",\"doi\":\"10.2478/abm-2022-0023\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong><i>Staphylococcus aureus</i> is a pathogen endemic in India and sometimes deadly for patients in intensive care units.</p><p><strong>Objectives: </strong>To determine the antibiotic-resistance pattern, biofilm forming ability, and clonal type of <i>S. aureus</i> from isolates collected in Tamil Nadu (south) and the Mizoram (northeast) regions of India.</p><p><strong>Methods: </strong>We collected <i>S. aureus</i> isolates from diagnostic laboratories in Tamil Nadu and Mizoram. An antibiotic susceptibility test was performed according to Clinical Laboratory and Standards Institute methods. Antibiotic-resistant determinants such as <i>mecA</i>, <i>mecC</i>, <i>blaZ</i>, <i>vanA</i>, <i>vanB</i>, and <i>vanC</i> were confirmed by polymerase chain reaction (PCR). All isolates were further studied for biofilm forming ability. Enterobacterial repetitive intergenic consensus (ERIC)-PCR was used for clonal analysis.</p><p><strong>Results: </strong>A study of 206 clinical isolates showed 52.9% prevalence of methicillin-resistant <i>S. aureus</i> in Tamil Nadu and 49.4% in Mizoram. Minimum inhibitory concentration tests showed a high prevalence of 67% oxacillin resistance in isolates from Tamil Nadu and 49% in isolates from Mizoram. PCR showed 53% <i>mecA</i> in Tamil Nadu and 49% <i>mecA</i> in Mizoram. Vancomycin-intermediate resistance <i>S. aureus</i> (VISA) prevalence was lower in isolates from Tamil Nadu (4%) and Mizoram (5%). All methicillin-resistant <i>S. aureus</i> (MRSA) isolates formed biofilms. Clonal analysis revealed a genetic relatedness between the isolates.</p><p><strong>Conclusions: </strong>The prevalence of MRSA is high in the regions studied, with most of the clinical isolates being multidrug resistant. Adopting appropriate community-based preventive measures and establishing antimicrobial stewardship is highly recommended to minimize the dissemination in antibiotic resistance.</p>\",\"PeriodicalId\":8501,\"journal\":{\"name\":\"Asian Biomedicine\",\"volume\":\"16 4\",\"pages\":\"191-199\"},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2022-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321179/pdf/\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Asian Biomedicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2478/abm-2022-0023\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Asian Biomedicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2478/abm-2022-0023","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
Antibiotic resistance, biofilm forming ability, and clonal profiling of clinical isolates of Staphylococcus aureus from southern and northeastern India.
Background: Staphylococcus aureus is a pathogen endemic in India and sometimes deadly for patients in intensive care units.
Objectives: To determine the antibiotic-resistance pattern, biofilm forming ability, and clonal type of S. aureus from isolates collected in Tamil Nadu (south) and the Mizoram (northeast) regions of India.
Methods: We collected S. aureus isolates from diagnostic laboratories in Tamil Nadu and Mizoram. An antibiotic susceptibility test was performed according to Clinical Laboratory and Standards Institute methods. Antibiotic-resistant determinants such as mecA, mecC, blaZ, vanA, vanB, and vanC were confirmed by polymerase chain reaction (PCR). All isolates were further studied for biofilm forming ability. Enterobacterial repetitive intergenic consensus (ERIC)-PCR was used for clonal analysis.
Results: A study of 206 clinical isolates showed 52.9% prevalence of methicillin-resistant S. aureus in Tamil Nadu and 49.4% in Mizoram. Minimum inhibitory concentration tests showed a high prevalence of 67% oxacillin resistance in isolates from Tamil Nadu and 49% in isolates from Mizoram. PCR showed 53% mecA in Tamil Nadu and 49% mecA in Mizoram. Vancomycin-intermediate resistance S. aureus (VISA) prevalence was lower in isolates from Tamil Nadu (4%) and Mizoram (5%). All methicillin-resistant S. aureus (MRSA) isolates formed biofilms. Clonal analysis revealed a genetic relatedness between the isolates.
Conclusions: The prevalence of MRSA is high in the regions studied, with most of the clinical isolates being multidrug resistant. Adopting appropriate community-based preventive measures and establishing antimicrobial stewardship is highly recommended to minimize the dissemination in antibiotic resistance.
期刊介绍：
Asian Biomedicine: Research, Reviews and News (ISSN 1905-7415 print; 1875-855X online) is published in one volume (of 6 bimonthly issues) a year since 2007. [...]Asian Biomedicine is an international, general medical and biomedical journal that aims to publish original peer-reviewed contributions dealing with various topics in the biomedical and health sciences from basic experimental to clinical aspects. The work and authorship must be strongly affiliated with a country in Asia, or with specific importance and relevance to the Asian region. The Journal will publish reviews, original experimental studies, observational studies, technical and clinical (case) reports, practice guidelines, historical perspectives of Asian biomedicine, clinicopathological conferences, and commentaries
Asian biomedicine is intended for a broad and international audience, primarily those in the health professions including researchers, physician practitioners, basic medical scientists, dentists, educators, administrators, those in the assistive professions, such as nurses, and the many types of allied health professionals in research and health care delivery systems including those in training.
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