印度南部和东北部金黄色葡萄球菌临床分离株的抗生素耐药性、生物膜形成能力和克隆谱分析。

IF 0.4 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Archana Loganathan, Ramesh Nachimuthu
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引用次数: 1

摘要

背景:金黄色葡萄球菌是印度的一种地方性病原体,有时对重症监护病房的患者是致命的。目的:确定在印度南部泰米尔纳德邦和东北部米佐拉姆邦分离的金黄色葡萄球菌的耐药模式、生物膜形成能力和克隆类型。方法:从泰米尔纳德邦和米佐拉姆邦的诊断实验室采集金黄色葡萄球菌分离株。根据临床实验室和标准协会的方法进行抗生素敏感性试验。聚合酶链反应(PCR)证实了耐药决定因子如mecA、mecC、blaZ、vanA、vanB和vanC。进一步研究了所有分离菌株的生物膜形成能力。克隆分析采用肠杆菌重复基因间一致性(ERIC)-PCR。结果:206株临床分离株的研究显示,泰米尔纳德邦耐甲氧西林金黄色葡萄球菌的患病率为52.9%,米佐拉姆邦为49.4%。最低抑菌浓度试验显示,泰米尔纳德邦和米佐拉姆邦分离株的oxacillin耐药率分别为67%和49%。PCR显示泰米尔纳德邦有53%的mecA,米佐拉姆邦有49%的mecA。泰米尔纳德邦(4%)和米佐拉姆邦(5%)的金黄色葡萄球菌(VISA)感染率较低。所有耐甲氧西林金黄色葡萄球菌(MRSA)分离株形成生物膜。克隆分析显示分离株间具有遗传亲缘关系。结论:MRSA在研究地区的流行率较高,大多数临床分离株具有多重耐药。强烈建议采取适当的社区预防措施并建立抗菌素管理制度,以尽量减少抗生素耐药性的传播。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Antibiotic resistance, biofilm forming ability, and clonal profiling of clinical isolates of <i>Staphylococcus aureus</i> from southern and northeastern India.

Antibiotic resistance, biofilm forming ability, and clonal profiling of clinical isolates of <i>Staphylococcus aureus</i> from southern and northeastern India.

Antibiotic resistance, biofilm forming ability, and clonal profiling of clinical isolates of Staphylococcus aureus from southern and northeastern India.

Background: Staphylococcus aureus is a pathogen endemic in India and sometimes deadly for patients in intensive care units.

Objectives: To determine the antibiotic-resistance pattern, biofilm forming ability, and clonal type of S. aureus from isolates collected in Tamil Nadu (south) and the Mizoram (northeast) regions of India.

Methods: We collected S. aureus isolates from diagnostic laboratories in Tamil Nadu and Mizoram. An antibiotic susceptibility test was performed according to Clinical Laboratory and Standards Institute methods. Antibiotic-resistant determinants such as mecA, mecC, blaZ, vanA, vanB, and vanC were confirmed by polymerase chain reaction (PCR). All isolates were further studied for biofilm forming ability. Enterobacterial repetitive intergenic consensus (ERIC)-PCR was used for clonal analysis.

Results: A study of 206 clinical isolates showed 52.9% prevalence of methicillin-resistant S. aureus in Tamil Nadu and 49.4% in Mizoram. Minimum inhibitory concentration tests showed a high prevalence of 67% oxacillin resistance in isolates from Tamil Nadu and 49% in isolates from Mizoram. PCR showed 53% mecA in Tamil Nadu and 49% mecA in Mizoram. Vancomycin-intermediate resistance S. aureus (VISA) prevalence was lower in isolates from Tamil Nadu (4%) and Mizoram (5%). All methicillin-resistant S. aureus (MRSA) isolates formed biofilms. Clonal analysis revealed a genetic relatedness between the isolates.

Conclusions: The prevalence of MRSA is high in the regions studied, with most of the clinical isolates being multidrug resistant. Adopting appropriate community-based preventive measures and establishing antimicrobial stewardship is highly recommended to minimize the dissemination in antibiotic resistance.

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来源期刊
Asian Biomedicine
Asian Biomedicine 医学-医学:研究与实验
CiteScore
1.20
自引率
0.00%
发文量
24
审稿时长
6-12 weeks
期刊介绍: Asian Biomedicine: Research, Reviews and News (ISSN 1905-7415 print; 1875-855X online) is published in one volume (of 6 bimonthly issues) a year since 2007. [...]Asian Biomedicine is an international, general medical and biomedical journal that aims to publish original peer-reviewed contributions dealing with various topics in the biomedical and health sciences from basic experimental to clinical aspects. The work and authorship must be strongly affiliated with a country in Asia, or with specific importance and relevance to the Asian region. The Journal will publish reviews, original experimental studies, observational studies, technical and clinical (case) reports, practice guidelines, historical perspectives of Asian biomedicine, clinicopathological conferences, and commentaries Asian biomedicine is intended for a broad and international audience, primarily those in the health professions including researchers, physician practitioners, basic medical scientists, dentists, educators, administrators, those in the assistive professions, such as nurses, and the many types of allied health professionals in research and health care delivery systems including those in training.
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